Study on chemical constituents from seeds of Trigonella foenum-graecum / 中草药

Objective: To research the chemical constituents from Trigonella foenum-graecum. Methods: The chemical constituents were separated and purified by sephadex LH-20, silica gel, semi-prepared HPLC and other chromatography techniques. Their structures were elucidated by their physicochemical properties and NMR data. Results: Six steroidal saponins and flavonoids (1-6) were isolated from the ethanol extracts of T. foenum-graecum and identified as 22-methoxy-trigoneoside IIb (1), gitogenin (2), diosgenin (3), luteolin (4), cynaroside (5), and luteolin-7-O-rutinoside (6). Conclusion: Compound 1 is a new compound, and compounds 5 and 6 are obtained for the first time from T. foenum-graecum.