RESUMEN
OBJECTIVE: To establish an HPLC-MS/MS method for determination of ASC-J9 in rat blood. METHODS: After liquid-liquid extraction, ASC-J9 was separated on a Symmetry Cl8 colume, with mobile phase of acetonitrile-water containing 0.1% formic acid and 10 mmol·L-1 ammonium for mate. The flow rate was 1.0 mL·min-1, mass shunt was 0.4 mL·min-1, and column temperature was main tained at 35℃. Quantification was performed in positive ion multiple-reaction-monitoring (MRM) mode. RESULTS: The calibration curve of ASC-J9 had good linearity in the concentration range of 3.54-1180 ng·mL-1. The extraction recovery rate was within 83.19% to 87.27%, and the intra-day and inter-day RSDs were both less than 8.89%. CONCLUSION: This method is specific, sensitive and suitable for determination of ASC-J9 in rat blood.