Genetic diversity of germplasm resources of Lonicera japonica by AFLP analysis / 中国中药杂志

<p><b>OBJECTIVE</b>This study aimed to analyze the genetic diversity and genetic relationship of germplasm resources of Lonicera japonica in main producing areas of China and provide reference for developing new varieties of L. japonica.</p><p><b>METHOD</b>Using 6 primer combinations, 13 germplasm of L. japonica were analyzed by AFLP marker. The genetic distance was worked out by using DPS V3.01 software, and the cluster was conducted based on UPGMA.</p><p><b>RESULT</b>A total of 435 bands were obtained including 191 polymorphic ones. The average polymorphic frequency was 43.9%. Cluster analysis showed that the relationship of cultivated variety from the same genuine area was near, and the classification result based on AFLP marker of germplasm of L. japonica from Shandong province was basically consistent with those on their morphological character.</p><p><b>CONCLUSION</b>AFLP marker can indicate the abundant genetic diversity of L. japonica and provide theoretical evidence for reasonable utilization and breeding new cultivar of L. japonica in molecular level.</p>

Studies on micromorphological characters of fruit coats in cultivated Fructus Trichosanthis in Shandong Province / 中国中药杂志

<p><b>OBJECTIVE</b>To explore the affinity relation among land race of Trichosanthes kirilowii and to provide evidence tor the classification and authentication.</p><p><b>METHOD</b>Using scanning electron microscope, characteristics of the pericarp surface were studied comparatively.</p><p><b>RESULT</b>The pericarp surface of the cultivated Fructus Trichosanthis in Shandong showed the characteristics of generality and diversity.</p><p><b>CONCLUSION</b>The results of the study could be used for the identification of the cultivated Fructus Trichosanthis in Shandong.</p>