Effect of total glycosides from Ranunculus japonicus on vasculogenic mimicry of human gastric cancer MGC803 cell line and its mechanism / 中草药

Objective: To investigate the effect of total glycosides from Ranunculus japonicus (TGRJ) on vasculogenic mimicry of human gastric cancer MGC803 cell line in vitro and its mechanisms. Methods: The effect of TGRJ on the cell adhesion of MGC803 was observed by cell attachment assay. The effect of TGRJ on the cell migration of MGC803 was studied by cell wound healing assay. The function of TGRJ on the tube-like structures formation of MGC803 in vitro was verified by tube formation assay. The effect of TGRJ on the expression of vasculogenic mimicry related genes was assessed by semi-quantitative RT-PCR assay. Results: TGRJ effectively inhibited the cell adhesion, cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry in vitro in a dosage-dependent manner. Furthermore, the semi-quantitative RT-PCR showed that TGRJ markedly inhibited the expression of MGC803 vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5. Conclusion: TGRJ could effectively inhibit the cell attachment and cell migration of MGC803, and the MGC803-mediated vasculogenic mimicry by down regulating vasculogenic mimicry-related genes VE-cadherin, sema 4D, and integrin β5.

Three-dimensional finite element analysis of the change of cracks in the cracked first mandibular molar under different loading conditions / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To study the change of the cracks and the influence on overall displacement of the cracked first mandibular molar under different loadings.</p><p><b>METHODS</b>Three-dimensional finite element models of first mandibular molar with cracks of different depth and length and a control model with no crack were created firstly. Then six loading conditions were applied to the models simulating the real mastication. The changes of the cracks and displacement of the teeth under the six loadings conditions were obtained by finite element method.</p><p><b>RESULTS</b>The length and depth of the cracks increased, the cracks of the occlusal surface become wider, and the crack was the widest under the fourth loading condition. Moreover, the edge of the cracks was irregular. The adjacent nodes were not in the same plane. The crack of the distal surface was the widest under the sixth loading condition. Compared to the teeth without cracks, the overall displacement of the teeth with cracks increased, but the increment was limited.</p><p><b>CONCLUSION</b>The change of the cracks is closely related to the initial crack forms and loading conditions.</p>