Molecular analysis of asymmetric somatic cell hybrids between carrot and Bupleurum scorzonerifolium / 中草药

Object Four regenerated somatic cell hybrids between carrot and Bupleurum scorzonerifolium Willd. proved by chromosome analysis were analyzed at the molecular level. Methods RAPD analysis and 5S rDNA spacer sequence were adopted. Results In the electrophoresis pattern of the PCR products the four somatic cell hybrids had the characteristic bands of two parents and new bands. Conclusion Their hybrid nature was clarified at molecular level. This provides a firm foundation to further analyze the main active components, saikosaponin of somatic cell hybrids, and screen out efficacious hybrid cell lines.

Tissue Culture of Chuanxizhangyacai(Swertia mussotii)Ⅰ.Induction and Maintenance of the Callus / 中草药

Calli were induced from young leaves,immature seeds and hypocotyl of Swertia mussotii Franch.Three kinds of media,MS,B5 and N6 were set up in the experiment. Calli were established with the highest frequency on to MS medium cantaining 3mg/L 2,4-D and 0. 6mg/L KT.The induction antmaintenence of the calli were affected markedly by the basic media and compound 2,4-D and KT in the media which proved that asextual reproduction of S. mussotii is possible by tissue culture