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Rev. Soc. Bras. Med. Trop ; 52: e20190135, 2019. graf
Artículo en Inglés | LILACS | ID: biblio-1041502

RESUMEN

Abstract INTRODUCTION: Musca domestica is resistant to many insecticides; hence, biological control is a suitable alternative. METHODS: We evaluated the lethality of strain Btk176 towards the larval and adult M. domestica and the histopathological effects in the larvae midgut. RESULTS: We observed 99% larval and 78.9% adult mortality within 48 hours of spore ingestion (dosage, 2.4×108 CFU/ml). The histopathological effects were consistent with cytotoxicity. PCR analysis showed the presence of the cry1Ba gene. Transmission electron microscopy revealed a bipyramidal parasporal body. Thurigiensin activity was not detected. CONCLUSIONS: The serovar, Btk176 might be a potential biocontrol agent for houseflies.


Asunto(s)
Animales , Bacillus thuringiensis , Toxinas Bacterianas/farmacología , Moscas Domésticas/efectos de los fármacos , Insecticidas/farmacología , Larva/efectos de los fármacos , Recuento de Colonia Microbiana , Control Biológico de Vectores/métodos , Reproducibilidad de los Resultados , Análisis de Varianza , Microscopía Electrónica de Transmisión , Exotoxinas
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