RESUMEN
Urea soluble antigens of Trichinella spiralis infective larva were prepared and the efficiency of the antigens in diagnosis of trichinosis by ELISA are studied. Specific IgG antibodies are detected by ELISA using urea soluble antigens in 91.67%(22/24) of 24 sera and 91.67% (ll/12)of 12 blood spots of filter paper of patients of trichinosis. Specific IgM antibodies are seen in 95.24% (20/21) of 21 sera of patients using the antigens. Sera of patients infected with Schistosoma japonicum have some cross reactions on the antigens. Sera from mice infected with - Schistosoma japonicum, Ancyclostoma caninum larva, and Plasmodium yoelii are negative. The urea soluble antigen has a relatively high sensitivity, specificity and good reproducibility in diagnosis of trichinoisis by ELISA, and has a practical and economic value.
RESUMEN
The infection of Clonorchis sinensis in two rhesus monkeys (Macaca mulatta, RH30 and RH48) was established by orally giving the metacercariae of the parasite (from Pseudorasbora parva from Sichuan) . Another monkey (RH405) served as control. The two monkeys, RH30 and RH48, were killed 30 and 48 days after infection respectively. The recovery rate of the parasite was 9.9% in RH30 and 31.59% in RH48. The worms recovered were the mature form, and mature eggs were found in the feces of the 2 monkeys 27-30 days after infecation.The pathological findings of clonorchiasis in the early stage in rhesus monkeys were eosinophilia (30 days after infection) , intralesional plasma cell infiltration (48 days after infection) , and adenomatous hyperplasia of the biliary epithelium in associatoin with an increase of acid mucin production. The significance of the last manifestation in relation to the stone formation was discussed.