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Objective:To translate the English version of the Modified Atrial Fibrillation Information Overload Scale into Chinese,and to preliminarily test its reliability and validity.Methods:According to the Brislin translation model,the English version of the Modified Atrial Fibrillation Information Overload Scale was translated into Chinese, and then was back-translated and modified for cultural adaptation followed by the guidelines. From December 2021 to May 2022,200 patients with atrial fibrillation in the Affiliated Hospital of Qingdao University and Qingdao Municipal Hospital Affiliated to Qingdao University were included by a convenience sampling method for a questionnaire survey, in order to evaluate its reliability and validity.Results:The Chinese version of the Modified Information Atrial Fibrillation Overload Scale contained 8 items. The Cronbach α coefficient of the Chinese version of the Modified Atrial Fibrillation Information Overload Scale was 0.884. The test-retest reliability was 0.653. The split-half reliability was 0.794. The scale-content validity index was 0.98, and the item-content validity index ranged from 0.83 to 1.00. Confirmatory factor analysis showed that the two-factor model fitted well, χ2/ df was 3.958, root mean square error was 0.026, comparative fit index was 0.936, goodness of fit index was 0.918, normal of fit index was 0.917, Tucker-Lewis index was 0.900, root mean square error of approximation was 0.08. Conclusions:The Chinese version of the Modified Atrial Fibrillation Information Overload Scale has good reliability and validity. It provides a reliable research tool for the patients to measure information overload in China, and provides a basis for health education for medical staff.
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Objective:To explore the relationships among perceived social constraints, social participation, as well as anxiety and depression in first stroke patients, and to further analyze the mediating effect of social participation on these variables.Methods:A total of 216 first stroke patients in the Department of Neurology of Affiliated Hospital of Qingdao University from April to December 2020 were recruited by convenience sampling and investigated by general information questionnaire, Social Constraints Scale (SCS), Chinese version of Impact on Participation and Autonomy Questionaire (IPA-C) and Hospital Anxiety and Depression Scale (HADS).Results:The total score of SCS, IPA-C, depression and anxiety were 33.49 ± 6.81, 43.42 ± 9.62, 8.05 ± 4.15 and 8.61 ± 2.59. Social constraints were positively correlated with social participation as well as anxiety ( r=0.644, 0.383, both P<0.05). Social constraints were positively correlated with social participation as well as depression ( r=0.482, 0.371, both P<0.05). The quality of social participation partially mediated the relationship between social constraints and anxiety (intermediary effect was 0.119), and also partially mediated the relationship between social constraints and depression (intermediary effect was 0.270). Conclusions:First stroke patients experience high level of social constraints, low quality of social participation and severe anxiety and depression. Social constraints can affect anxiety and depression through social participation. Medical staff should build a good environment to meet the needs of stroke patients of social participation, help patients to establish a correct psychological coping style, reduce patients' avoidance of social participation due to perceived constraints and exclusion, and thus promote the mental health of patients.
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Objective@#To compile and evaluate the questionnaire of knowledge, belief and practice of perioperative nurses in the prevention of deep venous thrombosis (DVT) in order to provide a tool for further investigation of the status quo of nursing care in the prevention of DVT during perioperative period.@*Methods@#Based on the theoretical model of knowledge, belief and practice, a questionnaire item pool was established by literature reading and semi-structured interviews, and an initial questionnaire was formed by Delphi expert inquiry. It was convenient to select 197 nurses engaged in surgery and operating room nursing in Qingdao Municipal Hospital Affiliated to Qingdao University to carry out the pre questionnaire, and to carry out the differentiation levels,relevance coefficient analysis and reliability and validity test were used to form a formal questionnaire.@*Results@#After two rounds of Delphi expert inquiries, three items with lower assignment value and four items with modified contents were deleted to form the initial questionnaire. The positive coefficients of experts in two rounds of inquiry were 85.00% and 100.00%, respectively; the Kendall's harmonious coefficients of experts were 0.195 and 0.204 (P<0.01); and the authoritative coefficients of experts were 0.836 and 0.849. Four items were deleted from 197 pre-survey questionnaires by discriminatory analysis, correlation coefficient analysis and reliability and validity test. The Item Level Validity (I-CVI) of the total questionnaire was 0.800-1.000, and the Scale Level Validity (S-CVI) was 0.825. In the structural validity analysis, the KMO value of each item was 0.824, the Bartlett spherical test value was 3170.304, P < 0.01. Twelve common factors with eigenvalues greater than 1 were extracted, and the cumulative variance contribution rate was 66.11%. The intrinsic consistency Cronbach a coefficient of the total questionnaire was 0.859, and the retest reliability was 0.706. Finally, a formal questionnaire with 40 items in 15 knowledge dimensions, 10 belief dimensions and 15 behavior dimensions was formed.@*Conclusion@#The reliability and validity of the questionnaire are well evaluated, and it can be used as a tool for investigating the knowledge, belief and practice of perioperative nurses in preventing deep venous thrombosis.
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Objective:To analyze the risk factors of medical adhesive-related skin injury (MARSI) in preterm infants to establish a nomogram model to predict the risk of MARSI.Methods:From July to September 2018, 268 premature infants who were hospitalized in NICU were enrolled in this study. Their clinical data were analyzed and univariate analysis was used to detemine the risk factors related to MARSI, The significant variables were included in the multivariate logistic regression analysis to analyze the independent risk factors for MARSI. Then the R software was used to establish a predictive nomogram model, Bootstrap method was used to validate the nomogram model and the consistency test of the correction curve was used to explore the predictive efficacy of the model in predicting the MARSI.Results:A total of 64 cases out of 268 premature infants had MARSI. The prevalence rate was 23.9 % (64/268). Birth age (28 to 32 weeks) ( P value was 0.021, OR value was 2.736, 95 % CI 1.163-6.435), edema ( P<0.01, OR value was 33.782, 95 % CI 10.510-108.583), maternal diabetes ( P value was 0.039, OR value was 16.011, 95 % CI 1.146-223.692), easy to tear tape ( P value was 0.027, OR value was 13.567, 95 % CI 1.340-137.311) were all independent risk factors for MARSI. The nomogram model showed a conformance-index of 87.29 %, while the consistency test of the correction curve showed that the prediction probability is consistent with the actual occurrence probability. Conclusions:The nomogram built based on the indexes of fetal age, skin state, maternal disease, mucous product has good discrimination and accuracy which could be helpful for screening the patients with high risk, with potentially high clinical application value.
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Objective:To compile and evaluate the questionnaire of knowledge, belief and practice of perioperative nurses in the prevention of deep venous thrombosis (DVT) in order to provide a tool for further investigation of the status quo of nursing care in the prevention of DVT during perioperative period.Methods:Based on the theoretical model of knowledge, belief and practice, a questionnaire item pool was established by literature reading and semi-structured interviews, and an initial questionnaire was formed by Delphi expert inquiry. It was convenient to select 197 nurses engaged in surgery and operating room nursing in Qingdao Municipal Hospital Affiliated to Qingdao University to carry out the pre questionnaire, and to carry out the differentiation levels,relevance coefficient analysis and reliability and validity test were used to form a formal questionnaire.Results:After two rounds of Delphi expert inquiries, three items with lower assignment value and four items with modified contents were deleted to form the initial questionnaire. The positive coefficients of experts in two rounds of inquiry were 85.00% and 100.00%, respectively; the Kendall's harmonious coefficients of experts were 0.195 and 0.204 ( P<0.01); and the authoritative coefficients of experts were 0.836 and 0.849. Four items were deleted from 197 pre-survey questionnaires by discriminatory analysis, correlation coefficient analysis and reliability and validity test. The Item Level Validity (I-CVI) of the total questionnaire was 0.800-1.000, and the Scale Level Validity (S-CVI) was 0.825. In the structural validity analysis, the KMO value of each item was 0.824, the Bartlett spherical test value was 3170.304, P < 0.01. Twelve common factors with eigenvalues greater than 1 were extracted, and the cumulative variance contribution rate was 66.11%. The intrinsic consistency Cronbach a coefficient of the total questionnaire was 0.859, and the retest reliability was 0.706. Finally, a formal questionnaire with 40 items in 15 knowledge dimensions, 10 belief dimensions and 15 behavior dimensions was formed. Conclusion:The reliability and validity of the questionnaire are well evaluated, and it can be used as a tool for investigating the knowledge, belief and practice of perioperative nurses in preventing deep venous thrombosis.
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Objective:To understand the status quo of perception control, self-management and cancer-related fatigue (CRF) in breast cancer patients receiving chemotherapy, and to investigate the impacts of perception control and self-management on CRF.Methods:In this study, 246 breast cancer patients undergoing chemotherapy from Shandong Cancer Hospital and Institute were investigated by means of cross-sectional survey. Cancer experience and efficiency scale was used to evaluate the patients′ perception control; cancer patients′ self-management scale was used to assess their self-management; and Piper Fatigue Scale was used to evaluate their CRF. Finally, the scores of CRF among the patients with different demographic data were compared, and the effects of perception control and self-management behavior on CRF were analyzed.Results:In univariate analysis, there were statistically significant differences of CRF scores resulting from different educational backgrounds ( F=3.392, P=0.019), sources of medical cost ( F=4.368, P=0.005), disease stages ( F=4.376, P=0.005), chemotherapy periods ( F=3.865, P=0.010) and courses of disease ( F=3.094, P=0.028). The differences in each dimension of perceived control cancer experience ( F=7.248, P=0.001), control efficacy ( F=96.595, P<0.001), self-management level of cancer patients ( F=65.009, P<0.001) and CRF ( F=130.973, P<0.001) were statistically significant. Cancer experience in perception control was positively correlated with CRF ( r=0.467, P<0.001); control effectiveness and self-management of cancer patients were negatively correlated with CRF ( r=-0.505, P<0.001; r=-0.564, P<0.001). Multiple linear regression showed that source of medical expenses (setting commercial insurance as the reference group), chemotherapy cycle (setting chemotherapy cycle ≥ 6 cycles as the reference group), cancer experience, control effectiveness, and self-management were entered in regression models, which could explain 55.5% of the total variation in CRF scores, and there was a significant linear relationship ( F=17.100, P<0.001). Conclusion:Medical staff should focus on CRF in patients at their own expense and in the 2-5 chemotherapy cycles. Cancer experience is positively correlated with CRF. Control effectiveness and self-management behavior are negatively correlated with CRF.
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Objective To investigate the relationship of stigma, coping style and psychosocial adjustment in young patients with breast cancer after operation, and the mediating effect of different coping style between stigma and psychosocial adjustment. Methods The general information questionnaire, Social Impact Scale (SIS), Medical Coping Style Questionnaire (MCMQ) and psychosocial adjustment questionnaire for breast cancer were used to investigate the stigma, coping style and psychosocial adjustment of 292 young patients with breast cancer after operation in the affiliated hospital of Qingdao University. Results There was a negative correlation between stigma and confrontation coping, psychosocial adjustment(r=-0.687--0.519, all P＜0.01), but a positive correlation with yield coping and avoidance coping (r=0.426-0.500, all P＜0.01).There was a positive correlation between psychosocial adjustment and confrontation coping(r=0.594, P＜0.01), and was a negative correlation between psychosocial adjustment and avoidance copying, yield copying(r=-0.643--0.519, all P＜0.05). The mediating effects of confrontation coping and avoidance coping were 32.86% and 18.81% respectively. Conclusions The stigma and coping style of young patients with breast cancer after operation were closely related to psychosocial adjustment. Nursing staff should guide the patients to adopt positive coping style, reduce the stigma, promote the psychosocial adjustment, and help the patients to return to normal life as soon as possible.
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Objective This study examined the effect of mindfulness-based cognitive behavioral therapy on the social psychology of women with human papillomavirus (HPV) infection. Methods Sixty women with HPV infection who visited a health center were randomly assigned to either an intervention (30 cases) or control group (30 cases), from December 2018 to March 2019. The control group received routine health education and psychological intervention. On the basis of the control group, the intervention group received 8 weeks of mindfulness-based cognitive therapy. The HPV Impact Profile (HIP) was used as an evaluation tool to compare the efficacy of the two groups. Results There were no significant baseline differences between the two groups. After intervention, HIP scores for worries and concerns, affective impact, sexual impact, and partner and communication were lower in the intervention group compared with the control group: worries and concerns (31.87±9.94 vs. 44.80±7.89), affective impact (30.93±9.79 vs. 54.93±12.89), sexual impact (27.60±10.73 vs. 58.87±8.35), partner and communication (28.90±8.57 vs. 46.83±12.2). There was a statistically significant difference (P<0.05) in each domain. Conclusion Mindfulness-based cognitive behavioral therapy can significantly improve negative emotions and sexual satisfaction among women with HPV infection.
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Objective@#To examine the integrated model of self-management behavior in middle-aged stroke patients based on health belief model and planned behavior theory.@*Methods@#A self-designed general information questionnaire, self-management behavior cognition and attitude questionnaire and self-management behavior scale, were used to investigate 654 cases of first middle-aged stroke patients in the neurology department of 3 grade a hospitals in Qingdao.@*Results@#The standardization factor load of each theoretical dimension in the theoretical integration model was 0.51-0.91 (0.72±0.11). Behavioral intention had a direct positive predictive effect on self-management behavior, with an effect value of 0.49 (t = 7.41, P < 0.01). Perceived behavior control not only had a direct positive prediction effect on self-management behavior, but also indirectly influenced self-management behavior through behavioral intention. The effect values are 0.12 (t = 2.75, P < 0.05) and 0.04 (t = 2.18, P < 0.05), respectively. Behavioral attitude exerts influence on self-management behavior through behavioral intention, and the effect value is 0.40 (t = 14.71, P < 0.01). Subjective norms not only influenced self-management behavior through behavioral intention, but also indirectly influenced behavioral intention and self-management behavior through behavioral attitude, with the effect value of 0.25. The effects of perceived benefits and perceived severity on self-management behaviors were 0.21 and 0.04, respectively. The theoretical integration model can explain 62% variance variation of self-management behavior.@*Conclusion@#The theoretical integration model of self-management behavior of middle-aged stroke patients based on the health belief model and planned behavior theory can better explain the behavior. This model can be applied to stroke patients in an attempt to provide theoretical basis for the formulation of personalized nursing measures for stroke patients.
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Objective@#To investigate the relationship of stigma, coping style and psychosocial adjustment in young patients with breast cancer after operation, and the mediating effect of different coping style between stigma and psychosocial adjustment.@*Methods@#The general information questionnaire, Social Impact Scale (SIS), Medical Coping Style Questionnaire (MCMQ) and psychosocial adjustment questionnaire for breast cancer were used to investigate the stigma, coping style and psychosocial adjustment of 292 young patients with breast cancer after operation in the affiliated hospital of Qingdao University.@*Results@#There was a negative correlation between stigma and confrontation coping, psychosocial adjustment(r=-0.687--0.519, all P<0.01), but a positive correlation with yield coping and avoidance coping (r=0.426-0.500, all P<0.01).There was a positive correlation between psychosocial adjustment and confrontation coping(r=0.594, P<0.01), and was a negative correlation between psychosocial adjustment and avoidance copying, yield copying(r=-0.643--0.519, all P<0.05). The mediating effects of confrontation coping and avoidance coping were 32.86% and 18.81% respectively.@*Conclusions@#The stigma and coping style of young patients with breast cancer after operation were closely related to psychosocial adjustment. Nursing staff should guide the patients to adopt positive coping style, reduce the stigma, promote the psychosocial adjustment, and help the patients to return to normal life as soon as possible.
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Objective To examine the integrated model of self-management behavior in middle-aged stroke patients based on health belief model and planned behavior theory. Methods A self- designed general information questionnaire, self- management behavior cognition and attitude questionnaire and self-management behavior scale, were used to investigate 654 cases of first middle-aged stroke patients in the neurology department of 3 grade a hospitals in Qingdao. Results The standardization factor load of each theoretical dimension in the theoretical integration model was 0.51-0.91 (0.72±0.11). Behavioral intention had a direct positive predictive effect on self-management behavior, with an effect value of 0.49 (t=7.41, P<0.01). Perceived behavior control not only had a direct positive prediction effect on self-management behavior, but also indirectly influenced self-management behavior through behavioral intention. The effect values are 0.12 (t=2.75, P<0.05) and 0.04 (t=2.18, P<0.05), respectively. Behavioral attitude exerts influence on self- management behavior through behavioral intention, and the effect value is 0.40 (t = 14.71, P < 0.01). Subjective norms not only influenced self-management behavior through behavioral intention, but also indirectly influenced behavioral intention and self-management behavior through behavioral attitude, with the effect value of 0.25. The effects of perceived benefits and perceived severity on self-management behaviors were 0.21 and 0.04, respectively. The theoretical integration model can explain 62% variance variation of self-management behavior. Conclusion The theoretical integration model of self-management behavior of middle-aged stroke patients based on the health belief model and planned behavior theory can better explain the behavior. This model can be applied to stroke patients in an attempt to provide theoretical basis for the formulation of personalized nursing measures for stroke patients.
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Objective To understand the job well-being status of ICU nurses,and to explore the mediating role of psychological capital in job stress and job well-being.Methods ICU nurses'general data questionnaire,the Nurse Questionnaire Psychological Capital,Chinese Nurses' Work Stressor Scale and Work Well-Being Scale were used to investigate 224 ICU nurses in three grade a general hospitals.Results Total score of psychological capital for ICU nurses was(4.46±0.55)points,the total average working pressure was(2.14±0.37)points,the happiness score was(4.36±0.67)points.Psychological capital and job well-being were positively correlated(r=0.513,P＜0.01),job stress and job well-being was negatively correlated(r =-0.454,P＜ 0.05).The structural equation results showed that psychological capital played an intermediary role in the sense of ICU nurses job stress and job happiness.Conclusions Hospital nursing managers should pay more attention to the mental health of ICU nurses and lower their work stress,so as to improve the work well-being of ICU nurses and improve the quality of ICU nursing.
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BACKGROUND:Currently, vertebral compression fractures are the most common osteoporotic fracture in postmenopausal women;however, incidence of refracture has aroused increasing attention due to a lack of standard treatment. OBJECTIVE:To evaluate whether vertebroplasty combined with anti-osteoporosis treatment can reduce refracture rate fol owing osteoporotic vertebral compressive fractures. METHODS:Eighty-nine patients with osteoporotic vertebral compressive fractures undergoing vertebroplasty were divided into control group (n=38) and treatment group (n=51) after making an informed choice about treatment. Chest/lumbar X-ray and bone mineral density determinations were performed through outpatient or inpatient fol ow-up. The spinal stability, bone mineral density and refracture rate of patients in both groups were fol owed up. RESULTS AND CONCLUSION:Seventy-eight patients achieved complete fol ow-up (ranged from 6-39 months, average 26.73 months). There was no significant difference in the spinal stability between both groups (P>0.05), while rare bone trabecula was found in the control group. There was a significant difference in bone mineral density between both groups at postoperative 12, 24, and 36 months (P<0.05). The refracture rate in the treatment group was significantly lower than that in the control group (P<0.05). Our results indicate that anti-osteoporosis treatment can effectively reduce the incidence of refracture after vertebroplasty in patients with osteoporotic vertebral compressive fractures, and this study found satisfactory short-and medium-term clinical outcomes.
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Objective To investigate the graduate students' mental pressure after the full im-plementation of the charging system and explore effective countermeasures of alleviating the psycho-logical pressure. Methods 413 full-time graduate students of Grade 2014 were randomly selected in a university of Shandong province. The graduate students' psychological pressure scale and self-designed questionnaire survey were used. The data were analyzed using SPSS 17.0 software, statistical descrip-tion of the survey data, independent sample t test, ANOVA. Results ①The psychological pressure of full-time graduate students at their own expense was in themedium pressure level; ②The economic pressure and academic pressure of the male graduate students were higher than those of the female graduate students (P=0.000,P=0.042), the rural economic pressures were greater than urban students graduate students graduate students (P=0.000); Professional graduate economic pressures were greater than academic graduate students (P=0.027);There is difference in the pressure of interpersonal commu-nication among different majors (P=0.016); ③78.75% (315/400) of the graduate students tuition fees, living expenses were mainly from their parents and the national and school awards; ④The percentage of those who completely agreed with the following view: full charge than at public expense is more advantageous to graduate education, comprehensive charge is advantageous to the education career development and comprehensive charge embodies the fairness of education was 84.75% (339/400), 71.25% (285/400) and 68.00% (272/400). Conclusion The full-time graduate students at their own expense have a more rational understanding of the charging policy; economic and academic pressures are the two outstanding pressure sources of the graduate students at the present stage. Graduate should improve their ability to cope with stress, and government and schools should take various measures to help students relieve stress.
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BACKGROUND:To grasp the optimal multiplicity of infection (MOI) and the time when stronger fluorescence intensities produce can lay the foundation for tracing observation of human bone marrow mesenchymal stem celsin vivo in animal models. OBJECTIVE:To investigate the feasibility of HIV-1 lentivirus carrying enhanced red fluorescent protein to transfect human bone marrow mesenchymal stem cels. METHODS:Human bone marrow mesenchymal stem cels at passage 4 were divided into blank group and MOI 2, 3, 4 groups. After that, the cels were seeded into 12-wel plates at a density of 5.0×105 , and cultured in 1 mL complete medium for adult bone marrow mesenchymal stem cels containing 1% fetal bovine serum. The infectious titer of lentivirus-carried enhanced red fluorescent protein was adjusted to 1.0×10 11 TU/L. Lentivirus solution 10, 15, 20 μL at MOI=2, 3, 4 were respectively added into the MOI 2, 3, 4 groups, and 10μL PBS was added into the blank group. At 24 and 72 hours after transfection, the expression of red fluorescence was observed under an inverted fluorescence microscope and the transfection efficiency was calculated. RESULTS AND CONCLUSION:Enhanced red fluorescent protein expressed stably in bone marrow mesenchymal stem cels. At 24 hours after transfection, red fluorescence could be seen under the inverted fluorescence microscope and achieved the peak at 72 hours after transfection. Within 21 days after transfection, there were no differences in the number of human bone marrow mesenchymal stem cels between the MOI 2, 3, 4 groups and blank group (P > 0.05). These results show that the HIV-1 lentivirus carrying enhanced red fluorescent protein is feasible to transfect human bone marrow mesenchymal stem cels, with the highest transfection efficiency when the MOI=4, which can express at least for 21 days and have no effects on the proliferative activity of labeled cels.
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To investigate the effects of salvianolic acid B (SA-B) on cardiovascular endothelial cell function and platelet activation during myocardial ischemia-reperfusion in rabbits.
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AIM: To investigate the intervention effect of astragaloside on adhesion of polymorphonuclear neutrophils (PMN) and vascular endothelial cells and on expression of nuclear factor-?B (NF-?B) in human umbilical vein endothelial cells (hUVECs) injured by hypoxia/reoxygenation due to ischemia/reperfusion. METHODS: The experiment was performed at the Cell Bioengineering Laboratory of Institute of Cerebrovascular Diseases, Affiliated Hospital of Medical College, Qingdao University from September 2005 to May 2006. ①Neonatal umbilical cords were offered by Department of Gynecology and Obstetrics in Affiliated Hospital of Medical College of Qingdao University, with informed consent of puerperant and their families. The experiment was accorded with ethical standard of Helsinki declaration. ②The hUVECs were cultured to the future passage. After the third passage, hUVECs were randomly divided into three groups. Cells in a control group were cultured under normal conditions; Cells in a hypoxia/reoxygenation group were cultured under in closed container with hypoxia for 1 hour, and then under normal conditions for 1 hour; The hUVECs in a astragaloside group were pretreatment by different doses of astragaloside (20, 40, 80 mg/L). After 12 hours, hUVECs suffered from hypoxia/reoxygenation. ③The concentration of intercellular adhesion molecule (ICAM)-1 and content of malondialdehyde (MDA) in supernatant were detected by enzyme linked immunosorbent assay (ELISA) and thiobarbituric acid method. Expression of NF-?B of hUVECs was analyzed by immunohistochemistry. PMN adhesion to hUVECs was measured by rose Bengal staining. RESULTS: ①Compared to the control group, the content of MDA remarkably increased in hypoxia/reoxygenation group (P
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Objective: To investigate the intervention effect of astragaloside on the expression of nuclear factor??(NF-?B) injured by hypoxia/reoxygenation(H/R) in vascular endothelial cells.Methods: The human umbilical vein endothelial cells(hUVECs) were cultured and passaged.After the third generation,hUVECs were randomly divided into three groups: control group,H/R group and astagaloside group.The cells in the control group were cultured under normal conditions;the cells of H/R group were cultured under hypoxia,in a closed container with oxygen concentrations below 1% for 1 hour,and then under normal conditions for 1 hour in a CO2 foster box;the hUVECs in astragaloside group were pretreated by different dose of astragaloside(the final concentrations of 20,40,80 mg/L) at the time of pre-hypoxia,after 12 hours of the pretreatment,they suffered from H/R.The concentration of intercellular adhesion molecule-1(ICAM-1) and content of malondialdehyde(MDA) in supernatant were detected respectively by ABCenzyme linked immunosorbent assay(ELISA) and thio-barbituric acid method.The expression of NF-?? of the vascular endothelial cells was analyzed by immunohistochemistry.Results: Compared with the control group,the content of MDA and concentration of ICAM1 in the supernatant fluid were remarkably increased in the H/R group(MDA content(6.98?1.15) ?mol/L vs.(2.38?0.49) ?mol/L,ICAM-1 concentration(3 169.01?132.75) ng/L vs.(995.14?74.93) ng/L,both P