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AIM: To explore the effect of microRNA-34a (miR-34a) on androgen receptor gene (AR) targeting regulation and proliferation and apoptosis of prostate cancer (PCa) LNCaP cells. METHODS: Thirty-six patients with PCa confirmed by prostate biopsy in urology department of our hospital were collected from October 2016 to September 2019, and 41 cases of benign prostatic hyperplasia (BPH) tissue samples were taken and operated at the same time. LNCaP cells were cultured in vitro and transfected with miR-34a mimics (mimics group), miR-34a mimic NC (NC group) respectively, and normal growth cells were set as blank control group (BC group). In addition, on the basis of mimics group, the AR over-expression vector (AR over-expression group) and its control group (AR control group) were transfected. The cell activity was detected by CCK-8 kit, the apoptosis rate was detected by Annexin V-FITC/PI double staining flow cytometry test kit, the expression of miR-34a and AR mRNA was detected by real-time quantitative PCR (RT-qPCR), the expression of AR protein, CyclinD1, c-Myc and apoptosis-related proteins (Bcl-2, Bax, capase-3) was detected by Western blotting. RESULTS: Compared with BPH tissues, the expression of miR-34a in PCa tissues decreased significantly (P<0.05), and the expression of AR mRNA and protein increased significantly (P<0.05). Compared with BC and NC group, miR-34a expression level, apoptosis rate and Bax protein expression level of LNCaP cells in mimics group increased significantly (P<0.05), the expression levels of AR, Cyclin D1, c-Myc and Bcl-2 decreased significantly (P<0.05), the activity of LNCaP cells decreased significantly at the same time (P<0.05). The dual-luciferase assay showed that miR-34a may have a direct targeting relationship with AR. Over-expression of AR gene could reverse the inhibition of miR-34a mimics on LNCaP cell proliferation and promote apoptosis. CONCLUSION: miR-34a may inhibit the proliferation and promote the apoptosis of prostate cancer LNCaP cells by regulating AR gene.
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Objective To investigate the prevalence of bladder hyperactivity in Huzhou city,Zhejiang prov-ince.Methods 1 872 patients aged over 50 years old male residents were selected as research subjects in Huzhou. To investigate the prevalence of bladder hyperactivity in the elderly male population in the local area.The correlation between age,IPSS score,body mass index,diabetes and the bladder was investigated.Results In 1 872 cases,the data were complete and in accordance with the requirements of this study were 1 863 cases.1 863 cases,505 patients with bladder hyperactivity,bladder disease prevalence was 27.1%.Bladder hyperactivity patients with IPSS score (17.9 ±8.1 )points,prostate volume (36.6 ±18.5 )mL,QOL score (3.6 ±1.1 )points,residual urine volume (26.9 ±3.5 )mL in the bladder,were higher than those with non bladder hyperactivity,IPSS score (3.2 ± 16.8)points,prostate volume (28.1 ±1.3)mL,QOL score (2.3 ±1.8)points,the differences were statistically sig-nificant (t =7.277,5.910,19.814,2.406,P <0.01 -0.05).Qmax(12.6 ±6.3)mL/s in the patients with bladder hyperactivity was significantly lower than Qmax(17.1 ±7.4)mL/s of non bladder hyperactivity,and the difference was statistically significant (t =3.577,P <0.01).With the increasing age of male population,the prevalence of blad-der diseases was increased.With the increase of IPSS score,the incidence rate of bladder excessive activity increased. Diabetes,higher body mass index,the incidence of bladder excessive activity was higher.Conclusion Men with blad-der hyperactivity disorder has higher prevalence in Huzhou city,Zhejiang province.Age,lower urinary tract symptoms, diabetes,obesity are the risk factors in the incidence of bladder disease.