Evaluation of the effects of sodium hypochlorite disinfection on alginate impression by immersion and spraying methods

Difficulties in sterilizing impressions by traditional methods have led to chemical disinfections as an alternative, and some studies have shown that some methods may adversely affect impressions. This study evaluated the effectiveness of 5.25% sodium hypochlorite spray and immersion disinfection methods on irreversible hydrocolloid impressions [Iralgin alginate]. In this study, twenty normal individuals were randomly selected. Impressions were made by a sterile tray from the maxillary arch and then were contaminated with the following organisms: staphylococcus aureus, streptococcus mutans, beta-hemolytic streptococcus type A, Pseudomonas aeruginosa, Escherichia coli and Candida albicans. Impressions were cut and divided into three sections anterioposteriorly and longitudinally. Then, sections were randomly placed in three groups as 1] control [without disinfection], 2] immersion and 3] spray. Then, the provided samples [pieces] of impression materials were cultured and microbial count of the three groups were compared. All of agar models from 20 alginate pieces in control group showed a positive microbial growth. Ten agar models form 20 alginate pieces which were disinfected by spraying method, showed also positive microbial growth and only 2 agar models out of 20 alginate pieces which were disinfected by immersion method showed a positive microbial growth. The mean number of organisms recovered form the surface of the impressions in control group was 3.52><10[8] CFU/ml while in spay and immersion groups it was 132217 CFU/ml and 1915 CFU/ml respectively. According to the results of this study, disinfection by immersion method with 5.25% sodium hypochlorite was more effective than the spray method

Comparison of the antibacterial effects of persica and matrica, two herbal mouthwashes with chlohexidine mouthwash

Fixed orthodontic appliances are considered to be a clinical risk factor in terms of gingival and enamel integrity, because of plaque accumulation around the bracket bases which increases the levels of microorganisms. Although chlorohexidine significantly reduces plaque accumulation, but its side effects prevents its common use. The purpose of this in vivo study was to compare the antibacterial effects of two herbal mouthwashes of persica and matrica with chlorhexidine in fixed orthodontic patients. A total of 68 orthodontic patients of 13-19 years old [who referred to a private clinic in 2002] with good oral hygiene were randomized into 4 groups. Group 1 [control] used water, group 2 used Chlorhexidine, group 3 used Persica and group 4 used matrica, twice a day for 3 weeks. The number of all bacterial colonies around the ring elastics of the upper canine and premolar growing in the culture media were counted before [T[1]] immediately after [T[2]] and 3 weeks after [T[3]] the use of the mouthwashes and water in all 4 groups and analyzed. Chlorhexidine, Persica and Matrica mouthwashes significantly reduced the amount of bacteria around the brackets immediately and after 20 days after their use. The inter group comparison showed that persica and matrica at T[2] and T[3] had significant differences with chlorhexidine group. So, Chlorhexidine was the most effective antibacterial mouthwash in T[2] and T[3]. Persica and Matrica groups showed no significant differences both in T[2] and T[3], but had significant difference with the control group. The use of herbal mouthwashes such as Persica and Matrica in orthodontic patients can significantly reduce the amount of microorganisms around the bracket bases without any side effect such as tooth discoloration which is seen with Chlorhexidine

Comparison of the effect of deconex [solarsept], micro 10 and cidex in disinfecting dental instruments

Infection control is the main step of prevention in dentistry. Effects of disinfectant solution must be evaluated in this process. The aim of this study was to evaluate the disinfecting effect of Deconex [solarsept], Cidex, and Micro 10 on high and low speed handpiece surfaces after cavity preparation. In this experimental study, 105 specimens were obtained from surfaces of high and low speed handpieces after cavity preparation by sterile swab and were divided into 7 groups. Each group contained fifteen specimens which were carried to thioglycolate media. Samples were incubated and remained for 48 hours at 37°C. Microorganisms were cultured in different media, and the rate of microbial growth were calculated. Following the various stages of investigation, it was found that all specimens from the surfaces of high and low speed handpieces, after cavity preparation, were infected, but no microbial growth and colony formation were detected after disinfecting the surfaces by disinfectants solutions used in the study. By evaluation of various sampling stages and microbial cultures in this study, it was concluded that the three disinfectants were able to destroy specific kinds of microorganisms after cavity preparation procedures, if they were provided and used by manufacturer instructions in order to decrease infection transmission