RESUMEN
Objective: this study was carried out for identification of Eimeria spp isolated from poultry breeder farms in Iran by PCR
Design: pop-Gene Analysis
Animals: poultry breeder farms
Procedures: a total of 114 litter samples from poultry breeder farms without previous exposure to anticoccidial vaccine were collected randomly from relatively five different climate regions of Iran. DNA was extracted from oocysts of samples, using phenol- chloroform and proteinase-K. Four pairs of specific primers, designated from Internal Transcribed Spacer-l[ITSI] regions of ribosomal DNA of E. acervulina, E. brunetti, E. necatrix, and E. tend/a and one pairs of universal primer BSEF-BSER which amplify ITS 1 of different Eimeria were used in PCR assay. In tests on purified genomic DNA from all species of Eimeria isolated from infected samples, each of four primer pairs amplified the ITS! region of their respective target species only. The PCR products were analyzed by agarose gel electrophoresis
Results: DNA fragments in sizes of 320 pairs [E. acervulina], 311 pairs [E. brunette], 384 pairs [E. necatrix] and 287 pairs [E. tenella] were detected on agarose gel electrophoresis. Universal primer pairs also amplified ITS1 of five Eimeria which isolated from infected samples
Laboratory implications: the results of this study were showed that PCR technique is a conventional method, faster, technically easier and very cheaper than other methods to identify the Eimeria spp. Finally, this technique can be recommended to be a routine work in well equipped veterinary diagnostic labs in IRAN