RESUMEN
Objective: Accelerated atherosclerosis is associated with chronic hemodialysis and more than one half of deaths in dialysis patients are of cardiovascular etiology. The study was designed to assess the concentration of serum total homocysteine [t-Hcy] and cardiac troponin-T [cTn-T] for the early detection of cardiovascular disease in chronic renal failure [CRF] and also to clarify their possible role as risk factors for the development of atherosclerosis in these patients to prevent and/or delay complications and to find out if there is a relation between these atherogenic markers with lipid profile and renal functions
Subjects and Methods: Sixty patients divided into three groups, GI: 20 chronic kidney disease [CKD] patients under conservative treatment. GII: 20 CKD patients on regular hemodialysis, GIII: 20 CKD patients with cardiac complications, all together with twenty healthy age and sex matched control subjects. Cardiac troponin-T, total homocysteine as well as lipid profile [total cholesterol, triacylglycerols, high density lipoprotein cholesterol and low density lipoprotein cholesterol] and renal profile [serum creatinine, BUN and uric acid] were determined
Results: Serum cTn-T was highly significantly increased in the three studied groups by 242, 614 and 7757%, respectively as compared to controls. Meanwhile, t-Hcy was significantly increased [p<0.05] in GI and highly significantly increased [p<0.001] in GII and GIII when compared to control group. There was a significant negative correlation between cTn-T and t-Hcy in GI [r=-0.38], uric acid in GII [r=-0.37] and creatinine in GIII [r=-0.39]. Such abnormalities might contribute to accelerated atherosclerosis in CKD patients
Conclusions: Laboratory determination of serum cTn-T together with t-Hcy is strongly recommended for early detection of cardiovascular disease in CKD
RESUMEN
The production of alginate by a mucoid strain of Azotobacter vinelandii was studied in batch culture. Highest alginate yield was obtained using Jarman N 2,-free mineral medium supplemented with 4% sucrose and the pH was initially adjusted to 8.0. Maximum alginate yield and productivity were achieved at the late growth phase [4 days old cultures], when each flask containing 100m1 medium was inoculated with 5% [V/V] bacterial suspension of 36h old cultures and shaked at 250rpm