RESUMEN
Background: Graft-versus-host disease [GVHD] has been associated with non- human leukocyte antigen [HLA] gene polymorphisms after allogenic Hematopoietic stem cell transplantation [aHSCT]
Objective: This study aims to investigate the role of three polymorphisms in Nucleotide-binding Oligomerisation Domain [NOD2] gene on the occurrence / severity of acute GVHD among Egyptian population
Methodology: A total of 64 patients and their corresponding donors who underwent aHSCT from HLA-identical matched siblings were genotyped by polymerase chain reaction and restriction fragment length polymorphism [PCR-RFLP]. Results were confirmed by automated laser sequencer
Results: There was highly statistical significant association between NOD2 recipient l007fs allele and higher incidence of aGVHD p= 0.006. There was no significant association between NOD2 donor l007fs allele, G908R allele in donors and recipients, R702W in donors and recipients with incidence and severity of aGVHD
Conclusion: This research an original pilot study to demonstrate that the NOD2 SNP13 [Leu1007fs] is a possible risk factor for aGVHD among Egyptian population