Middle East Consensus Statement on the Prevention, Diagnosis, and Management of Cow's Milk Protein Allergy / 대한소아소화기영양학회지

Presented are guidelines for the prevention, diagnosis, and treatment of cow's milk protein allergy (CMPA) which is the most common food allergy in infants. It manifests through a variety of symptoms that place a burden on both the infant and their caregivers. The guidelines were formulated by evaluation of existing evidence-based guidelines, literature evidence and expert clinical experience. The guidelines set out practical recommendations and include algorithms for the prevention and treatment of CMPA. For infants at risk of allergy, appropriate prevention diets are suggested. Breastfeeding is the best method for prevention; however, a partially hydrolyzed formula should be used in infants unable to be breastfed. In infants with suspected CMPA, guidelines are presented for the appropriate diagnostic workup and subsequent appropriate elimination diet for treatment. Exclusive breastfeeding and maternal dietary allergen avoidance are the best treatment. In infants not exclusively breastfed, an extensively hydrolyzed formula should be used with amino acid formula recommended if the symptoms are life-threatening or do not resolve after extensively hydrolyzed formula. Adherence to these guidelines should assist healthcare practitioners in optimizing their approach to the management of CMPA and decrease the burden on infants and their caregivers.

Correction: Middle East Consensus Statement on the Prevention, Diagnosis, and Management of Cow's Milk Protein Allergy / 대한소아소화기영양학회지

Table 2 contains a typographical error.

Identification of non specific esterase isoenzyme patterns by iso-electric focus in acute leukemias

Forty-four individuals, 32 patients with acute leukemia [32 with ANLL and 7 with ALL] representing group and 12 subjects [8 healthy individuals were randomly selected as a control group and 4 with CLL]. From 8 healthy controls, purified granulocyte and monocyte preparations were isolated to identify the normal non-specific esterase isoe-lectric focusing [IEF] for each cell type. Four patients known to have CLL [3 with B-CLL and 1 with T - CLL] were chosen for preparation of B and T-lymphocytes and used as control lymphocyte pattern. To all patients complete hemogram, cytochemical stains for peripheral blood and bone marrow smears [myeloperoxidase, periodic acid shiff, specific and nonspecific esterases] were performed. Identification of nonspecific esterase isoenzyme pattern for each cytologic type of leukemia using isoelectric focusing of anaphthyl acetate esterase on polyacry-lamide gel [PAG] was also done. Our results showed that nonspecific esterase zymograms identified consistent and distinctive isoenzymatic patterns that were characteristic for each type of leukemia. This work showed that despite different isoenzyme patterns displayed by ALL zymograms, they were easily distinct from ANLL zymograms. Moreover, our results provided further evidence for the herte-rogeneity of ANAE in AML and AMML and that NaF inhibition of ANAE reactions have little diagnostic application in the assessment of monocytic involvement. Conversely, the use of NaF with IEF was found to be essential in the differentiation between various types of leukemia