Evaluation of norovirus rapid detection kit / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>Evaluating the accuracy and safety as well as the equivalence compared with the control kit of RIDA QUICK Norovirus detection kit(R-Biopharm, Germany).</p><p><b>METHODS</b>Based on the results of commercially available IDEA Norovirus detection kit (ELISA), the sensitivity and specificity and accuracy of RIDA QUICK Norovirus detection kit (immunochromatographic assay) were evaluated.</p><p><b>RESULTS</b>The sensitivity and specificity of RIDA QUICK Norovirus detection kit were 98.4% and 92.4%, and the accuracy was 97.6% compared with the control kit.</p><p><b>CONCLUSION</b>RIDA QUICK Norovirus detection kit has good sensitivity and specificity for the detection of norovirus antigens.</p>

The analysis of the test results in HIV screening laboratory of Beijing Friendship Hospital in 2008 / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>According to test results of the Hospital of AIDS screening laboratory in 2008, after counting analysis to assess the prevalence of AIDS, we can early detect positive cases in the future and effectively control the spread of AIDS.</p><p><b>METHODS</b>All serum samples were screened by ELISA method and we reexaminated the samples by PA. As long as one result is positive by the two methods, then we sent the positive samples to Beijing Center for Disease Control and Prevention by Western Blot method to confirm the result.</p><p><b>RESULTS</b>A total of 21 467 samples were detected and 29 (13.5% 0) were positive screening results. We confirm there were 7 (24.1%) positive samples and 12 (41.4%) suspected samples. We researched the epidemiology of the specimens by its source and age and sex.</p><p><b>CONCLUSION</b>Application of ELISA method for HIV screening test has a practical significance, it is accurate and fit to record the results of the screening test for AIDS.</p>

In vitro inhibitory mechanism of respiratory syncytial virus with solution prescription of zhidanhuayu / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study the effective part of solution prescription of Zhidanhuayu (ZDHY) against respiratory syncytial virus (RSV) in vitro.</p><p><b>METHODS</b>Observe the pathology of RSV to Hep-2 under the condition of different concentrations and each effective part of ZDHY.</p><p><b>RESULTS</b>The concentration limit causing celluar toxicity of ZDHY is 5.5 mg/ml. The ZDHY failed to block the absorption of RSV to Hep-2 within this concentration, and consequently the cell fell into the full pathological changes. During the concentration of 2.75-5.50 mg/ml, the ZDHY directly destroyed virus array,meanwhile, the infected cells that treated by the medicine kept healthy also.</p><p><b>CONCLUSION</b>ZDHY could not defend the infection of RSV, but is able to destroy the RSV directly and inhibit the RSV inhabiting in the cell.</p>

In vitro inhibition of respiratory syncytial virus with combined ribavirin and an oral preparation of traditional Chinese medicine Hu Fei / 中华实验和临床病毒学杂志

<p><b>OBJECTIVE</b>To study the in vitro antiviral effect of ribavirin combined with an oral preparation of traditional Chinese medicine "Hu Fei" (protecting the lung) on respiratory syncytial virus (RSV).</p><p><b>METHODS</b>Cytopathic effects (CEP) of RSV on Hep2 cells were observed after adding different concentrations of ribavirin, Hu Fei and combination of both into the culture medium.</p><p><b>RESULTS</b>The minimum concentration of ribavirin and Hu Fei for complete inhibition of CPE caused by RSV was 7.80 microg/ml and 5.00 mg/ml, respectively. When the combination of ribavirin and Hu Fei was applied, their minimum concentrations needed for complete inhibition were decreased to 0.98 ?g/ml and 0.63 mg/ml.</p><p><b>CONCLUSIONS</b>Both ribavirin and Hu Fei showed in vitro anti-RSV effect, but the inhibitory effect of combined ribavirin and Hu Fei was more potent than either of the preparation alone.</p>