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Journal of Experimental Hematology ; (6): 1429-1435, 2021.
Artículo en Chino | WPRIM | ID: wpr-922276

RESUMEN

OBJECTIVE@#To establish the in vivo traceable acute myeloid leukemia mice model with Luciferase-Expressing KG1a Cells.@*METHODS@#KG1a cells with stable luciferase gene expression (called as KG1a-Luc cells) were constructed by lentivirus transfection, then sifted out by puromycin. Eighteen male NOD-SCID-IL2rg@*RESULTS@#KG1a cells expressing luciferase stably were successfully obtained. The tumor luminescence wildly spread at day 17 captured by in vivo imaging. The KG1a-Luc tumor cells could be detected in the peripheral blood of the mice, with the average percentage of (16.27±6.66)%. The morphology and pathology result showed that KG1a-Luc cells infiltrate was detected in bone marrow, spleens and livers. The survival time of the KG1a-Luc mice was notably shorter as compared with those in the control group, the median survival time was 30.5 days (95%CI: 0.008-0.260).@*CONCLUSION@#The acute myeloid leukemia NOD-SCID-IL2rg


Asunto(s)
Animales , Masculino , Ratones , Modelos Animales de Enfermedad , Subunidad gamma Común de Receptores de Interleucina , Leucemia Mieloide Aguda , Luciferasas/genética , Ratones Endogámicos NOD , Ratones SCID
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