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Journal of Southern Medical University ; (12): 1947-1949, 2008.
Artículo en Chino | WPRIM | ID: wpr-321785

RESUMEN

<p><b>OBJECTIVE</b>To extract the plasma membrane proteins from mouse liver cells and investigate the approach for fractionating the protein mixtures by two-dimensional liquid chromatography.</p><p><b>METHODS</b>The plasma membrane of the liver cells from 10 mice was extracted by differential centrifugation and sucrose density-gradient centrifugation. The plasma membrane proteins were exchanged with the start buffer and separated by chromatofocusing in the first-dimensional fractionation. The final results were transformed into UV/pI maps using ProteoVue software.</p><p><b>RESULTS</b>We successfully extracted the plasma membrane proteins from mouse liver cells. Sixteen fractions between pH 8.5-4.0 were recovered in the first-dimensional chromatofocusing followed by 2D- chromatographic fractionation, and the results were displayed as UV/pI maps.</p><p><b>CONCLUSION</b>This approach for fractionating the mouse liver cell plasma membrane protein study provides the foundation for further studies on the functions of plasma membrane proteins and differential proteome of diseases.</p>


Asunto(s)
Animales , Masculino , Ratones , Membrana Celular , Metabolismo , Fraccionamiento Químico , Métodos , Cromatografía Liquida , Métodos , Hígado , Biología Celular , Proteoma , Metabolismo , Proteómica , Métodos
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