Phylogenetic analysis of theileria annulata infected cell line S15 Iran vaccine strain

Bovine theileriosis results from infection with obligate intracellular protozoa of the genus Theileria. The phylogenetic relationships between two isolates of Theileria annulata, and 36 Theileria spp., as well as 6 outgroup including Babesia spp. and coccidian protozoa were analyzed using the 18S rRNA gene sequence. The target DNA segment was amplified by PCR. The PCR product was used for direct sequencing. The length of the 18S rRNA gene of all Theileria spp. involved in this study was around 1,400 bp. A phylogenetic tree was inferred based on the 18S rRNA gene sequence of the Iran and Iraq isolates, and other species of Theileria available in GenBank. In the constructed tree, Theileria annulata [Iran vaccine strain] was closely related to other T. annulata from Europe, Asia, as well as T. lestoquardi, T. parva and T. taurotragi all in one clade. Phylogenetic analyses based on small subunit ribosomal RNA gene suggested that the percent identity of the sequence of Iran vaccine strain was completely the same as Iraq sequence [100% identical], but the similarity of Iran vaccine strain with other T. annulata reported from China, Spain and Italy determined the 97.9 to 99.9% identity

Thermostability and efficacy of 1-2 vaccine against Newcastle disease [ND] in Iran: a pilot study

Thermostable vaccine against Newcastle disease [ND] is a potential alternative to chicken vaccination in villages, since cold chain is not needed in this process. The purpose of this study was to investigate thermostability and efficacy of 1-2 vaccine against ND. The vaccine tested for sterility, intracerebral pathogenicity index [ICPI] and 50% embryo infectious dose [EID50]. The wet vaccine stored at 20°C for 1,2 and 4 weeks named A, B and D, respectively. Each preparation [10 [7.5] EID50] was used to inoculate twenty, 4-week-old chicks via eye drop. All groups except the control were vaccinated twice in 2 weeks interval. Haemagglutination inhibition test used for evaluation of the antibody response. Two weeks after the first vaccination, a peak titer of about log.2 [6] and log.2 [4.6] was observed in A and B groups, but D group did not induce any antibody response in their sera. All the vaccinated groups responded to vaccination after second trial and produced mean titers of log.2 [6.2], log.2 [6] and log.2 [5.4] were observed, respectively