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Rev. Soc. Bras. Med. Trop ; 51(1): 88-93, Jan.-Feb. 2018. tab
Artículo en Inglés | LILACS | ID: biblio-1041448

RESUMEN

Abstract INTRODUCTION: Here, we determined the genes encoding antibiotic resistance enzymes and virulence factors and evaluated the genetic relationship between Enterobacter spp. isolated from different clinical samples. METHODS: A total of 57 clinical isolates of Enterobacter spp. were tested for the production of extended-spectrum β-lactamases (ESBLs), carbapenemase, and AmpC using phenotypic and genotypic methods. RESULTS: The most common ESBLs and AmpC β-lactamases were bla TEM (63.3%) and bla EBC (57.7%), respectively. The most prevalent virulence gene was rpos (87.7%). The random amplified polymorphic DNA (RAPD) patterns of strains were genetically unrelated. CONCLUSIONS: RAPD polymerase chain reaction analysis revealed high genetic diversity among isolates.


Asunto(s)
Humanos , Proteínas Bacterianas/biosíntesis , Proteínas Bacterianas/genética , beta-Lactamasas/genética , Escherichia coli/efectos de los fármacos , Heces/microbiología , Antibacterianos/farmacología , Fenotipo , Proteínas Bacterianas/efectos de los fármacos , beta-Lactamasas/biosíntesis , Reacción en Cadena de la Polimerasa , Células Clonales , Farmacorresistencia Bacteriana Múltiple , beta-Lactamas/efectos adversos , Escherichia coli/enzimología , Escherichia coli/genética , Pruebas Antimicrobianas de Difusión por Disco , Genotipo , Irán
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