Effect of corn oil, olive oil, sheep's and cow's ghee on the expression of apoB protein in Syrian mice's intestine and liver

Background and Aims: type and saturation of fatty acids can have an important impact on the level of triglyceride, cholesterol, very low and low-density lipoproteins in the blood and thus affect the development of atherosclerosis. Saturated fatty acids have an additive effect on blood cholesterol while for unsaturated fatty acids, a lowering effect has been reported. Fatty acids can have different effects on lipoproteins metabolism and apolipoproteins expression because oils used by humans have different compositions. One of the important apolipoproteins is apolipoprotein B [apoB]. This study was conducted to compare the effect of different nutritious fats on expression of apoB protein

Materials and Methods: for this purpose, 48 Syrian male mice were selected and randomly divided into six groups of eight: chow, diet with 10% corn oil, diet with 10% olive oil, diet with 10% cow ghee, diet with 10% sheep ghee, and diet with 2% cholesterol. After two months, liver and intestine were removed and transferred into liquid nitrogen at -70degreeC. Protein was extracted and the expression of apoB was studied by western blotting

Results: an increase in the expression of intestinal apoB48 was identified in olive oil and cow ghee groups. Hepatic apoB100 expression was increased in the cholesterol group compared with the corn oil group

Conclusion: this study indicates that olive oil and cow ghee consumption increase intestinal apoB48 expression

Hepatoprotective effect of otostegia persica boiss. shoot extract on carbon tetrachloride-induced acute liver damage in rats

In this study, the hepatoprotective effect of the methanol extract of aerial parts [shoot] from Otostegia persica Boiss [Golder] was investigated against the carbon tetrachloride [CCl[4]]-induced acute hepatotoxicity in male rats. Liver damage was induced through the oral administration of 50% CCl[4] in liquid paraffin [2.5 mL/Kg bw, per os] 60 min after the administration of the methanol extract of O. persica shoot [in 200, 300, 400 mg/Kg bw doses] and assessed using biochemical parameters [plasma and liver tissue malondialdehyde [MDA], transaminase enzyme levels in plasma [aspartate transaminase [AST], alanine aminotransferase [ALT]] and liver glutathione [GSH] levels]. Results show that the methanol extract of O. persica shoot is active at 300 mg/Kg [per os] and it possess remarkable antioxidant and hepatoprotective activities. Additionally, histopathological studies verified the effectiveness of this dose of extract in acute liver damage prevention