RESUMEN
Satureja Khozestanica is among the native Iranian plants which grow mostly in Lorestan and Khozestan. A number of reports show that Satureja plant possesses anti-inflammatory effects. The aim of this study is to determine the effects of Satureja Khozestanica essential oil on nitric oxide in the presence or absence of lipopolysaccharide in J774A.1 macrophage-like cell line. J774A.1 cells are cultured at a concentration of 10[6] cells per ml in RPMI 1640 medium with 10% fetal Bovin serum. Then, the cells were treated in the presence of lipopolysaccharide [1micro g/ml] containing 0.004%, 0.008%, and 0.016% doses of Satureja essential oil and 0.004, 0.008, and 0.016 macromolar of carvacrol over 12, 24, and 48 hours. Next, the level of nitric oxide in the cell culture supernatant was measured using Griess's technique. The results showed that Satureja Khozestanica extract significantly decreased the production of nitric oxide in J774A.1 macrophage cell line, dependent on dose and time. In addition, in the presence of lipopolysaccharide, Satureja Khozestanica extract inhibited the production of nitric oxide more effectively than carvacrol. The results demonstrate that Satureja Khozestanica essential oil is effective in lowering inflammation through inhibition of nitric oxide production. Therefore, this extract may be effectual in treatment of inflammatory diseases
RESUMEN
The prevalence of diabetes is increasing around the world. Oxidative stress is one of the risk factors in diabetes. The purpose of this study is to examine the protective effects of the essential oil of Satureja khuzestanica on the level of serum lipids and atherogenic indices in the male rats with diabetes. This experimental study was conducted on thirty male rats randomly divided into three groups including: 1- control group, 2- rats with untreated diabetes 3- rats with treated diabetes [through orally intake of water containing 500 ppm of the essential oil of Satureja khuzestanica]. The second and third groups also developed diabetes through injection of alloxan tetrahydrate [120 mg/kg]. After eight weeks of treatment, their blood sample was taken, and then the levels of glucose, lipids and atherogenic indices of the serum were measured. The levels of glucose, triglyceride, cholesterol, low density lipoprotein [LDL], very low density lipoprotein [VLDL] and atherogenic indices of plasma in treated diabetic group were significantly decreased. The amount of high density lipoprotein [HDL] was significantly increased in the treated group. The results obtained from this study showed that the essential oil of Satureja khuzestanica has beneficial effects on the level of serum lipids and atherogenic indices of rats with type 1 diabetes