Effect of endometrial cell-conditioned medium and platelet-rich plasma on the developmental competence of mouse preantral follicles: An in vitro study / 대한생식의학회지

Objective@#The aim of this study was to evaluate the impacts of platelet-rich plasma (PRP) and conditioned medium (CM) derived from endometrial stromal cells on mouse preantral follicle culture in a two-dimensional system to produce competent mature oocytes for fertilization. @*Methods@#In total, 240 preantral follicles were isolated from female mouse ovarian tissue and divided into four groups. The preantral follicles were isolated three times for each group and then cultured, respectively, in the presence of alpha minimum essential medium (control), PRP, CM, and PRP+CM. The in vitro growth, in vitro maturation, and cleavage percentage of the preantral follicles were investigated. Immunocytochemistry (IHC) was also conducted to monitor the meiotic progression of the oocytes. Additionally, the mRNA expression levels of the two folliculogenesis-related genes (Gdf9 and Bmp15) and two apoptosis-related genes (Bcl2 and Bax) were investigated using real-time polymerase chain reaction. @*Results@#In the PRP, CM, and PRP+CM groups, the preantral follicle maturation (evaluated by identifying polar bodies) were greater than the control group. The cleavage rate in the CM, and PRP+CM groups were also greater than the control group. IHC analysis demonstrated that in each treatment group, meiotic spindle was normal. In the PRP+CM group, the gene expression levels of Bmp15, Gdf9, and Bcl2 were greater than in the other groups. The Bax gene was more strongly expressed in the PRP and control groups than in the other groups. @*Conclusion@#Overall, the present study suggests that the combination of CM and PRP can effectively increase the growth and cleavage rate of mouse preantral follicles in vitro.

[ effect of local anesthetic concentration and volume on the block onset time and success rate in infraclavicular approach]

Local anesthesia techniques are suitable for upper limb surgery but the effects of anesthetic agent's concentration, volume and total dose on motor and sensory block success rate is controversial. The aim of the present study was to investigate the effect of anesthetic agent's concentration and volume on the sensory and motor nerve block onset time and its quality in upper limb orthopedic surgeries. In this double blind clinical trial 60 patients ASA class I, II who were candidate for upper limb surgeries were enrolled to the study and were randomly divided into two groups; lidocaine 2% [7ml] and lidocaine 1.3% [10ml]. Vertical infra-clavicular technique using ultrasound guide was applied. Onset time and quality of sensory and motor block of Radial, Median, Ulnar and MSN nerves were recorded and compared every 5 minutes for 20 minutes. Onset time of sensory and motor block in the lidocaine 1.3% was significantly shorter than lidocaine 2% [p>0.05]. The success rate of sensory and motor block in group of lidocaine 1.3% was higher than lidocaine 2% but there was no significant difference between the two groups [82.8% and 78.6% respectively, p>0.05]. The quality of motor block of Radial nerve and sensory block of MSN were significantly better in the group of lidocaine 1.3%. The volume of anesthetic agents can affect the onset time of sensory and motor block of brachial plexus but there was no statistically significant effect on success rate of blockade