The male toad test for pregnancy diagnosis using Burmese toads

(1) Catheterise toad and examine for presence of spermatozoa.(2) Inject urine sample into dorsal lymph sac of toad.(3) Collect urine from toad every hour up to 4 hours and examine for spermatozoa.(4) Positive diagnosis indicated by presence of spermatozoa in any sample. (5) If no reponse, inject 50 I.U. Chorionic Gonadotropin and examine urine an hour later. Spermatozoa in urine indicate negative pregnancy teat.