Your browser doesn't support javascript.
loading
Mostrar: 20 | 50 | 100
Resultados 1 - 2 de 2
Filtrar
Añadir filtros








Intervalo de año
1.
Mem. Inst. Oswaldo Cruz ; 101(8): 875-880, Dec. 2006. ilus, graf
Artículo en Inglés | LILACS | ID: lil-440575

RESUMEN

Strains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterotoxin genes, and subsequent serotyping, but most clinical and public health laboratories do not have the capacity or resources to perform these tests. In this study, polyclonal rabbit and monoclonal mouse IgG2b antibodies against ETEC heat-labile toxin-I (LT) were characterized and the potential applicability of a capture assay was analyzed. IgG-enriched fractions from rabbit polyclonal and the IgG2b monoclonal antibodies recognized LT in a conformational shape and they were excellent tools for detection of LT-producing strains. These findings indicate that the capture immunoassay could be used as a diagnostic assay of ETEC LT-producing strains in routine diagnosis and in epidemiological studies of diarrhea in developing countries as enzyme linked immunosorbent assay techniques remain as effective and economical choice for the detection of specific pathogen antigens in cultures.


Asunto(s)
Humanos , Animales , Niño , Ratones , Conejos , Anticuerpos Monoclonales/biosíntesis , Toxinas Bacterianas/inmunología , Enterotoxinas/biosíntesis , Escherichia coli/inmunología , Inmunoglobulina G/biosíntesis , Anticuerpos Monoclonales , Enterotoxinas/genética , Enterotoxinas/inmunología , Escherichia coli/genética , Técnicas para Inmunoenzimas , Inmunoglobulina G , Reacción en Cadena de la Polimerasa , Reproducibilidad de los Resultados , Sensibilidad y Especificidad , Serotipificación
2.
Rev. microbiol ; 30(4): 365-8, out.-dez. 1999. ilus, graf
Artículo en Inglés | LILACS | ID: lil-286793

RESUMEN

The genetic diversity of 41 typical and atypical enteropathogenic Ëscherichia coli" (EPEC) strains of the serogroup O55 was analysed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on "E. coli" O55 infection


Asunto(s)
Escherichia coli/aislamiento & purificación , Escherichia coli/patogenicidad , Infecciones por Escherichia coli/diagnóstico , Infecciones por Escherichia coli/patología , Variación Genética/genética , Técnica del ADN Polimorfo Amplificado Aleatorio/normas
SELECCIÓN DE REFERENCIAS
DETALLE DE LA BÚSQUEDA