RESUMEN
Objectives: different chelators may be used during root canal treatment, offering various advantages including lubricant effect inside the canal and smear layer removal. However, chelator residues in narrow root canals can lead to apical microleakage. The aim of this in-vitro study was to compare apical microleakage following the use of three root canal chelators via fluid filtration method in root canals instrumented with ProTaper rotary system
Methods: sixty-eight distobuccal canals of maxillary first molars were randomly divided into six groups of four experimental [n=15] and a positive and a negative control group [n=4]. In groups one to three, RC-Prep, 17% EDTA and Glyde File Prep were used as chelators, respectively and sodium hypochlorite [NaOCl] was used as irrigant in all groups except for group four. In group four, root canals were instrumented without chelators and only saline was used for irrigation. Root canals in all groups were prepared using ProTaper rotary system up to F[2] file and filled using cold lateral condensation technique with gutta-percha and AH26 sealer. Apical microleakage was assessed by fluid filtration method. The data were subjected to Kruskal-Wallis test
Results: no significant differences were noted among the experimental groups regarding apical microleakage [p>0.05]. However, preparations with RC Prep+ NaOCl and Glyde File Prep+ NaOCl yielded the highest and the lowest values of apical microleakage, respectively
Conclusion: use of different chelators did not cause statistically significant difference in apical microleakage of root canals
RESUMEN
Antibacterial activity is one of the desirable properties of an ideal sealer. This study aimed to compare the antimicrobial effect of three different sealers, i.e. resin [AH26], calcium hydroxide [Apexit] and zinc oxide eugenol [ZOE] based sealers. Direct contact test with agar diffusion was used in this in vitro study. The freshly mixed sealers were AH26, Apexit and pure ZOE. They were prepared according to manufacturer's instruction and placed in prepared wells of 30 agar plates inoculated with Streptococcus [S] mutans and Prevotella [P] melaninogenicus [15 samples for each microorganism]. All plates were incubated for 7 days [196 hour] at 37C under anaerobic conditions, and zones of inhibition were measured after 3 days, 5 days and 7 days. The data were analyzed using Kruskal-Wallis and Friedman tests. In all determined intervals, the antibacterial activity of AH26 was significantly greater than other test materials [P<0.001]. ZOE sealer had moderate effect on test microorganisms, whilst Apexit showed the lowest antibacterial activity on S. Mutans and no antibacterial activity on P. melaninogenicus. The ascending sequence of bacterial growth inhibition zones was as AH26>Pure ZOE>Apexit