RESUMEN
Objective To explore the protective mechanism of Potentilla anserina polysaccharide (PAP) on acute liver injury induced by D-galactosamine (D-GlaN) in mice. Methods Sixty Kunming mice were randomly divided into normal control group, model group, bifendate group (positive control) and PAP (50, 100, 200 mg/kg) treated groups, with 10 mice in each group. After treatment with normal saline, bifendate and PAP (50, 100, 200 mg/kg) for 7 days, themice in normal control group were injected intraperitoneally with normal saline, and those in the other 5 groups were injected intraperitoneally with D-GlaN to establish acute liver injury models. All the animals were sacrificed 24 h after model establishment, and the levels of hepatic superoxide dismutase (SOD), malondialdehyde (MDA), glutathione (GSH), and glutathione peroxidase (GSH Px) were determined. Results Compared with normal control group, the model group had significantly increased hepatic MDA level (P<0. 05) and significantly decreased activities of hepatic SOD, GSH-Px and level of GSH (P<0. 05, P<0. 01). Compared with the model group, bifendate and 50, 100, 200 mg/kg PAP significantly decreased hepatic MDA level (P<0. 05), increased the activities of hepatic SOD, GSH-Px and level of GSH (P<0. 05, P<0. 01) in mouse acute liver injury model. Conclusion PAP can protect the liver of mice with acute liver injury induced by D-GlaN, which is probably through scavenging free radicals, protecting cell membranes and inhibiting lipid peroxidation.