RESUMEN
A method was established to analyze the fingerprint of Dazhu Hongjingtian capsule by HPLC-DAD.The separation was performed on Agilent Eclipse Plus-C₁₈(4.6 mm×250 mm, 5 μm) with methanol-0.1% formic acid solution as the mobile phase for gradient elution at a flow rate of 1.0 mL•min⁻¹; the detection wavelength was set at 276 nm and column temperature was set at 35 ℃. A total of 10 batches of samples were detected by the above method, and based on their fingerprint by using Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004A), 21 common chromatographic peaks were determined and after the individual common peak whose peak area was greater than 50% of the total peak area was deducted, the similarity results of these samples were analyzed and compared. The results showed that the similarity of 10 batches of samples was all higher than 0.940. HPLC/Q-TOF-MS was used to identify the common chromatographic peaks in the fingerprint and determine the molecular formulas of twenty-one common chromatographic peaks. The structures of 11 fingerprint peaks were tentatively identified based on the control products and mass spectrometry information. This was the first time to establish fingerprint by using HPLC method and identify fingerprint peaks by using HPLC/Q-TOF-MS. This method has good precision, stability and repeatability, and could provide basis for quality evaluation of Dazhu Hongjingtian capsule.
RESUMEN
Objective An arterial blood pressure fitting method, based on pulse wave signal and vessel elastic chamber model, was researched and implemented to meet the requirement of continuous blood pressure (BP) measurement in health care. Methods Photoplethysmography (PPG) signal, electrocardiograph (ECG) signal and BP data of the subjects were collected by a self developed wearable physiological monitoring system. In accordance with the temporal relation between ECG and PPG signals, the equation of regression analysis on systolic BP value and pulse wave transient time (PWTT) was deduced, and the diastolic BP measurement was achieved by coefficients analysis on PPG wave and parameter calculation on blood vessel single elastic chamber model. Results The experiment results showed that the mean difference and the standard deviation of the method were （0.51±0.74) kPa［(384±5.54) mmHg］, reaching the standard (0.665±1.064) kPa［(5±8) mmHg］ proposed by Association for the Advancement of Medical Instrumentation (AAMI). Conclusions Human blood pressure can be estimated by the pulse wave signal and elastic chamber model, which provides a new method for the continuous blood pressure measurement.