RESUMEN
【Objective】 To establish a high-throughput detection method for ABCG2*376T allele of Jr(a-), and apply it to the study of the frequency of this allele in the Chinese population. 【Methods】 The specific primers were designed and synthesized, the sample carrying homozygous ABCG2*376T alleles, obtained in the previous study, was used as the homozygous positive control, and the sample carrying heterozygous allele as the heterozygous positive control. The wild-type sample was used as a negative control, and a high-resolution melting curve(HRM) method for detecting this allele was established. The established method was used to screen DNA samples from blood donors in Guangzhou, and the samples carrying ABCG2*376T alleles were sequenced to confirm the accuracy of the HRM method. 【Results】 A HRM method, which can detect ABCG2*376T allele and accurately type homozygotes and heterozygotes at the same time, had been established successfully. Fifteen individuals with heterozygous alleles were screened out of 1 560 blood donors in Guangzhou, while none homozygous allele was detected. 【Conclusion】 The HRM method can be used to accurately screen and type ABCG2*376T allele. The frequency of this allele in Chinese population is about 0.48%(15/3120).
RESUMEN
Objective To investigate curative outcomes of the limited-incision surgery under cold light source for treating calcified lumbar disc protrusion. Methods A total of 36 patients with calcified lumbar disc protrusion underwent limited-incision surgery under cold light visualization from January 2000 to June 2005. The patients were maintained at a prone position. The operation was carried out under local anesthesia. A longitudinal skin incision around 3 cm in length was made. After the inferior border of the vertebral plate and the intervertebral space were exposed, a lamina retractor was inserted above the superior border of the articular process. Then a fenestration and a discectomy were performed under cold light visulization. Results The operative time was 75.3?3.5 min and the intraoperative blood loss was 100.6?5.5 ml. Cerebrospinal fluid leakage occurred in 3 patients. All the 36 patients were followed for 6~60 months (mean, 27 months). Symptoms of low back pain, lower limb pain, and intermittent limping disappeared completely in all the 36 patients. According to the Nakai standard, the rate of excellent or good results was 94.4% ( 34/36 ). Conclusions Limited-incision surgery under cold light source for the treatment of calcified lumbar disc protrusion offers advantages of simplicity of performance, minimal invasion, and satisfactory outcomes.