RESUMEN
Objective For analysis of the ability to resist the shear stress and anti-calcification of endothelial cells (EC), and analysis of migrating and self-repairing ability of myofibroblasts. Methods (1) The fresh bovine pericardial patches were acellularized, tanned and modified. (2) Autologous myofibroblasts and ECs were seeded onto the patches of Group A sequentially; Group B, unseeded group. Then the patches of both groups were implanted to the porcine abdominal aortic wall separately. (3) The retrieved specimens were sent for thickness, calcium content, scanning electron microscopy (SEM) and histological examination. Results (1) In Group A, white smooth tissue covered the surface of the specimens; In Group B, the colores of specimens was grey-yellow. The calcium in Group A was significantly less than in Group B (P