RESUMEN
<p><b>OBJECTIVE</b>To explore the therapeutic effects and mechanisms of Zhubi capsule on adjuvant arthritis (AA) in rats.</p><p><b>METHOD</b>The model of rat was induced by intradermal injection of CFA. The perimeter of ankle joint was measured at different time points. Con A-induced splencyte proliferation was examined by MIT assay and the expression of IL-1, IL-6 in synovium was determined by immunohistochemical method.</p><p><b>RESULT</b>After the preventive administration of Zhubi capsule (5.2, 2.6, 1.3 g x kg x d(-1), x 25 d), it was found that Zhubi capsule significantly inhibited the primary and secondary ankle joint swelling. The increased Con A-induced splencyte proliferation reaction and the activated IL1, IL-6 expression of AA rat were suppressed by the treatment with Zhubi capsule.</p><p><b>CONCLUSION</b>Zhubi capsule has a therapeutic effect on AA rats, and its mechanism is related to the immunoregulative function.</p>
Asunto(s)
Animales , Masculino , Ratas , Articulación del Tobillo , Patología , Artritis Experimental , Metabolismo , Patología , Cápsulas , Proliferación Celular , Combinación de Medicamentos , Medicamentos Herbarios Chinos , Farmacología , Interleucina-1 , Metabolismo , Interleucina-6 , Metabolismo , Materia Medica , Farmacología , Fluidez de la Membrana , Plantas Medicinales , Química , Ratas Wistar , Bazo , Biología Celular , Membrana SinovialRESUMEN
<p><b>OBJECTIVE</b>To compare the hemostatic effect of Flos Sophorae in crude, parched and carbonized forms and its extracts, including rutin, quercetin and tannin.</p><p><b>METHODS</b>All the testing samples were orally administered to the experimental animals for 5 days, then the bleeding time (BT), coagulation time (CT), platelet count and capillary permeability (CP) in the treated mice were tested, and the prothrombin time (PT), fibrinogen (FBG) content and platelet aggregation rate (PAR) in the treated rats were determined.</p><p><b>RESULTS</b>All the samples could lower the CP, BT and CT in mice and also decrease the plasma PT in rats. All three forms of Flos Sophorae could increase FBG in rats, while the three extracts of it could inhibit the PAR in rats obviously. In addition, rutin had the effect of raising the platelet count.</p><p><b>CONCLUSION</b>All the three forms and three extracts of Flos Sophorae have hemostatic effect, the effect of parched and carbonized form is higher than that of crude drug. The mechanism of the hemostatic effect of the six kinds of sample might be various.</p>