Effects of 200cH medications on mice bone marrow cells and macrophages. / Effects of 200cH medications on mice bone marrow cells and macrophages.

Paracelsus once wrote: "All things are poison and nothing is without poison, only the dose permits something not to be poisonous." Latter Hahnemann formulated the law of similars, preparations which cause certain symptoms in healthy individuals if given in diluted form to patients exhibiting similar symptoms will cure it. Highly diluted natural complexes prepared according to Hahnemann?s ancient techniques may represent a new form of immunomodulatory therapy. The lack of scientific research with highly diluted products led us to investigate the in vivo and in vitro actions of commonly used medications. Here we describe the results of experimental studies aimed at verifying the effects of Mercurius solubilis, Atropa Belladonna, Lachesis muta and Bryonia alba. All medications were at 200cH dilution. Animals were maintained for 7 days and were allowed to drink the medications, which were prepared in a way that the final dilution and agitation (200cH) was performed in drinking water. The medication bottle was changed and sucussed every afternoon. Coculture of non treated mice bone marrow cells and in vitro treated peritoneal macrophages were also performed. After animal treatment the bone marrow cells were immunophenotyped with hematopoietic lineage markers on a flow cytometer. We have determined CD11b levels on bone marrow cells after culture and co-culture with treated macrophages and these macrophages were processed to scanning electron microscopy. We have observed by morphological changes that macrophages were activated after all treatments. Mercurius solubilis treated mice showed an increase in CD3 expression and in CD11b on nonadherent bone marrow cells after co-culture with in vitro treatment. Atropa Belladonna increased CD45R and decreased Ly-6G expression on bone marrow cells after animal treatment. Lachesis muta increased CD3, CD45R and, CD11c expression and decreased CD11b ex vivo and in nonadherent cells from co-culture. Bryonia alba increased Ly-6G, CD11c and CD11b expression ex vivo and when in co-culture CD11b was increased in adherent cells as well as decreased in nonadherent cells. With these results we have demonstrated that highly diluted medications act on immune cells activating macrophages, and changing the expression profile of hematopoietic lineage markers. Highly diluted medications are less toxic and cheaper than other commonly used medications and based on our observations, it is therefore conceivable that this medications which are able to act on bone marrow and immune cells may have a potential therapeutic use in clinical applications in diseases were the immune system is affected and also as regenerative medicine as it may allow proliferation and differentiation of progenitor cells.

Modelos estatísticos preditores de pressão arterial de indivíduos da Região Metropolitana de Olinda-PE / Models for the analysis of arterial pressure both and Região Metropolitana de Olinda - Pernambuco- Brazil

Este estudo teve o objetivo de propor modelos estatísticos preditores dos níveis de pressão arterial sistólica e diastólica, em individuos do sexo masculino e feminino. Para as análises de regressão, foram selecionadas variáveis: idade (ID), frequência cardíaca de repouso (FCr), estatura (EST), sedentarismo (DS), não sedentarismo (NS), perímetros da cintura (CT), do quadril (QD), massa corporal total (MCT) para ambos os sexos e dobras cutâneas para homens: abdominal (AB), triciptal(TR) e subescapular (SE) e para mulheres: abdominal (AB), supraíliaca(SI) e coxa(CX), Utilisou-se o processo de Stepwise para selecionar as variáveis significativas aos modelos de regressão linear múltipla, com fator de transformação de Box e Cox(1964) simplificado, (p<0,05). Obteve-se uma alta correlação das variáveis: FCr,EST, CT, QD, MCT, para ambos os sexos e a ID só para o feminino. Os modelos estatísticos propostos, inferem sobre a necessidade de considerar as variáveis acima citadas, sobre as reais medidas de pressão arterial em indivíduos normotensos de ambos os sexos

Níveis urinários da proteína transportadora do retinol em uma populaçäo pediátrica: evoluçäoem funçäo da idade / Retinol-biding protein urinary levels in a pediatric population: evolution according to age

Objetivo: O rim da criança, em particular e do recém-nascido, é especialmente susceptível a injúrias isquêmicas e/ou tóxicas, que comprometem preferencialmente os túbulos renais. Assim, marcadores precoces e fidedignos da funçäo tubular renal seriam bastante úteis em Pediatria. A proteína transportadora de retinol utinária (RBPu) vem sendo utilizada com este propósito, funcionando como marcador de funçäo do túbulo contornado proximal (TCP). Nesse sentido, nosso objetivo foi avaliar o comportamento da RBPu em crianças para estabelecer um padräo de referência e avaliar possíveis diferenças entre as diversas faixas etárias. Métodos: Estudamos crianças normais, entre um mês de idade e 8 anos, e recém-nascidos normais, a termo (RNT) e pretermo (RNPT), estes últimos sem intercorrências além das inerentes à prematuridade, as quais näo determinaram repercussöes hemodinâmicas. Foram coletadas amostras isoladas de urina, sendo uma coleta para crianças acima de 1 mês e coletas periódicas abaixo desta idade. Nesta urina, além da realizaçäo de fita-teste...