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@#Objective To investigate the effect of cognitive behavioral multi-level intervention on post-stroke depression. Methods 160 patients with post-stroke depression in our hospital from February 2013 to February 2015 were divided into control group (n=80) and obser-vation group (n=80). Both groups received conventional medical treatment and nursing care. The observation group received cognitive be-havioral multi-level intervention nursing care, including personal psychology, behaviour and joint relationship. Self-rating Depression Scale (SDS) was assessed, and the serum level of interleukin (IL)-1β, IL-6 and tumor necrosis factor-α(TNF-α) were mesured before and after treatment. Results After treatment, the score of SDS was lower (t=2.705, P<0.05), and the levels of IL-1β(t=8.182), IL-6 (t=2.883), TNF-α(t=2.604) were lower in the observation group than in the control group (P<0.05). The incidence of depression was also lower in the observa-tion group than in the control group ( χ 2=6.144, P<0.05) after 6 months follow-up. Conclusion Cognitive behavioral multi-level interven-tion could effectively prevent post-stroke depression.
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Objective To observe the effects of differentially expressed peroxisome proliferatoractivated receptor γ (PPAR γ) on cell migration,invasion and proliferation of endometrial cancer cells.Methods Two endometrial cancer cell lines ECC-1 (ER positive) and KLE (ER negative) cells were used in this study.To up or down regulate PPARγ expression,the transient transfection by using PPARγ expression vector (PPARγ expression vector group) and PPARγ small interference RNA (PPARγ siRNA group) were done.The negative control groups were cells transfected by nonsence sequence siRNA (siRNA non sence sequence group) or empty vector (empty vector group).At the same time,cells only added with liposome were used as blank control group.Then,quantitative real time (RT)-PCR and western blot were used to detect PPARγexpression both in mRNA and protein levels.To assess the expression levels of Wnt signaling pathway,western blot was performed to analysis protein levels of β-catenin and C-myc.The effects on cell migration,invasion and proliferation using in vitro transwell migration,invasion assays and cell counting kit-8 (CCK-8) assay were further be examined.Results After transfection for 48 hours,quantitative RT-PCR and western blot showed that PPARγmRNA (5.18 ± 0.99,4.54 ± 0.89) and protein (1.45 ± 0.12,1.30 ± 0.13) expression levels significantly increased and the protein levels of β-catenin (0.44 ± 0.06,0.46 ± 0.04) and C-myc (0.42 ± 0.08,0.30 ± 0.11) decreased in PPAR γ expression vector group,while in PPARγ siRNA group,PPARγ mRNA (0.48 ± 0.08,0.53 ± 0.11) and protein (0.41 ±0.04,0.49 ±0.05) expression levels decreased and the protein levels of 3-catenin (1.18 ±0.12,0.89 ±0.07) and C-myc(0.91 ±0.08,0.77 ±0.12) increased significantly compared with control groups (all P ＜ 0.05).In vitro migration and invasion assay indicated that the migratory and invasive cell numbers of PPARγ expression vector group (ECC-1:129 ± 9,63 ± 12 ; KLE:119 ± 9,68 ± 16) were significantly decreased,while the migratory and invasive cell numbers of were PPARγ siRNA group (ECC-1:201 ± 14,142 ±9 ; KLE:170 ± 11,138 ± 7) increased significantly compared with those in control groups(all P ＜ 0.05).CCK-8 assay showed that A values (0.66 ±0.14,0.78 ±0.06) in PPARγexpression vector group were lower than those in control groups,and in PPARγ siRNA group,A values (1.42 ± 0.16,1.23 ± 0.04) were higher than those in control groups,and there were statistically significant difference among them (all P ＜ 0.05).Conclusion Up-regulated PPARγ gene expression could inhibit endometrial cancer cell migration,invasion and proliferation abilities,and down-regulated PPARγ gene expression could promote endometrial cancer cell migration,invasion and proliferation abilities.
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Objective To explore the application effect of cognitive behavioral intervention on chronic obstructive pulmonary disease nursing care.Methods 80 patients with chronic obstructive pulmonary disease were randomly divided into an experimental group and a control group(n=40).Both groups were given medical treatment and conventional nursing care,and the control group was given the cognitive behavioral intervention nursing care additionally.The average days of stay,the ratio of readmission,the treatment adherence,and smoking cessation rate in one year were recorded.Moreover,the life quality of the two groups was analyzed with a CAT assessment test.Results Compared with the control group,the experimental group achieved shorter average days of stay,higher medication adherence and smoking cessation rate,lower readmission rate and admission rate.The experimental group achieved better life quality with lower CAT value than the control group.Conclusion Cognitive behavioral intervention can obviously improve the treatment effectiveness of patients with chronic obstructive pulmonarv disease.
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Objective:To investigate the expressions of peroxisome proliferator-activated receptor gamma (PPARγ), estrogen re-ceptor alpha (ERα), and estrogen receptor beta (ERβ) in endometrial carcinoma and to analyze their correlations and clinical signifi-cance. Methods:Immunohistochemical assay and Western blot were used to detect the expressions of PPARγ, ERα, and ERβin normal endometrial tissues and well-differentiated, moderately differentiated, and poorly differentiated endometrial carcinomas. Results:PPARγexpression was significantly lower in endometrial carcinoma than in the normal endometrium and was intimately associated with cli-ni-copathologic variables. ERαexpression gradually decreased in moderately and poorly differenti-ated endometrial carcinomas. How-ever, no significant differences were found between the normal endometrium and well-differentiated endometrial carcinoma. ERβex-pression only decreased in the poorly differentiated endometrial carcinoma. No significant association was observed between ERβand clinicopathologic variables. Pearson correlation analysis showed a significant positive cor-relation between the expressions of PPARγand ERα. No correlations were observed between the expressions of ERαand ERβand between that of ERβand PPARγ. Conclusion:The expression lev-els of PPARγand ERαwere significantly associated with the clinicopathologic stage of endometrial carcinoma, and have essential functions in endometrial tumorigenesis and tumor progression.
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miR-205 is differentially expressed in tumor tissues and is closely related to tumorigenesis and tumor metastasis.miR-205 can regulate the biological behaviors of tumor cells such as cell proliferation,differentiation,in situ invasion and distant metastasis by post-transcriptional regulation through binding to targeted genes.Additionally,further research of miR-205 may be helpful for tumor diagnosis,targeted therapy and prognosis.
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MicroRNA is a class of non-protein coding,single-stranded small RNAs of 22-25 nucleotides length.It takes part in many physiological processes including tumorigenesis.In the present focus studies,molecular biology and bioinformatics methods are used to study the role of miRNA in tumors.Several miRNAs with research value have been found and their mechanisms have been investigated in endometrial carcinoma.Results from the studis provide valid evidence for further research.