RESUMEN
Objective: To study what are needed during the invasion of Staphylococcus aureus into cultured human osteoblasts.Methods: Mid-logarithmic growing Staphylococcus aureus 6571 was prepared. Human osteoblasts were cultured from bone explants. Chloramphenicol, rifampicin, novobiocin, cycloheximide, and monodansylcadaverine were used as inhibitors of either bacteria or cells to study their roles in the invasion of Staphylococcus aureus into osteoblasts. Results: Chloramphenicol, rifampicin, novobiocin, and monodansylcadaverine decreased the invasive ability of the bacteria; while cycloheximide increased the ability.Conclusion: De novo protein as well as RNA-and DNA-synthesis by the bacteria is necessary for invasion. The inhibition of osteoblast protein synthesis increases the uptake of the bacteria.