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Objective To investigate the expression of FoxO1 gene in idiopathic congenital talipes equinovarus(ICTEV) in Xinjiang Uigur and its correlastion with deformity degree of ICTEV.Methods The FoxO1 protein expression level of foot muscle tissues in 51 Uigur children cases of ICTEV and 25 Uigur normal children were detected by Western blot,the relative expression level of FoxO1 mRNA in different deformity degrees of ICTEV was detected by using real time fluorescence quantitative PCR.Results The Western Blot detection results showed that the expression level of FoxO1 protein in ICTEV children patients was (0.52 ± 0.03),which was lower than(1.61 ± 0.15) in normal children,the difference was statistically significant (P =0.017).The real time fluorescence quantitative PCR detection showed that the relative expression level of FoxO1 mRNA in the Dimeglio type Ⅱ,Ⅲ and Ⅳ were 1.02±0.24,0.67±0.15 and 0.24±0.19 respectively.There was negative correlation between the deformity degree of ICTEV and FoxO1 mRNA relative expression level.Conclusion The transcription level of FoxO1 gene is gradually decreased with the deformity degree aggravation of ICTEV,suggesting that the FoxO1 gene may involve in the whole onset process of Uigur ICTEV.
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Objective To study the effects of diabetes mellitus(DM)on fas receptor-induced cardiomyocytes apoptosis and inflammatory response in patients with acute myocardial infarction(AMI).Methods 70 patients with ST-elevated myocardial infarction were divided into two groups:without(n=36)and with diabetes mellitus(n=34).Strepavidin-biotin ELISA was used to examine the serum levels of sFas,sFasL,TNF-? and IL-6.Results As the concentrations of sFas and sFasL immediately after primary PCI were higher in patients of AMI with DM than without DM(4.18?0.86 vs 3.30?0.82 ng/ml,6.15?1.56 vs 4.34?1.37 ng/ml,respectively,P