Biomechanical effect of meniscus posterior horn in posterior cruciate ligament rupture patients undergoing arthroscope adjunctive treatment and clinical curative effect observation / 中国医师进修杂志

Objective To explore the clinical curative effect of arthroscope adjunctive treatment in patients with posterior cruciate ligament(PCL)rupture,and investigate the biomechanical attribute of meniscus posterior horn. Methods The clinical data of 49 posterior cruciate ligament rupture patients having underwent arthroscope adjunctive posterior cruciate ligament prothesis were retrospectively analyzed. The patients were followed up at least 6 months, and functional recovery of the knee was assessed. Biomechanical study was completed,which included 2 healthy adult knee specimens. The specimens were tested under 200 N loads at the condition of complete or incised posterior cruciate ligament to measure the strain of meniscus posterior horn.Results The operation time was 1.31-2.08 (1.56 ± 0.81)h,postoperative drainage volume was(69 ± 24)ml,and length of stay in hospital was 6-11(7.56 ± 2.18)d.The patients were followed up for 6-13(8.7 ± 2.9)months,and the Lysholm knee function score was 84 - 96 (91.40 ± 5.18) scores. There were no complications such as ligament reinjury,venous thrombosis and joint stiffness in all patients.The biomechanical test result showed that the strain variables of meniscus posterior horn in different angle(0°,30°,60°and 90°)were significantly increased after posterior cruciate ligament rupture:(27.51 ± 2.38)°vs.(18.12 ± 3.24)°,(41.23 ± 2.81)°vs. (12.58 ± 1.57)°,(67.56 ± 1.68)°vs.(24.56 ± 1.74)°and(79.61 ± 1.16)°vs.(34.65 ± 3.24)°,and there were statistical differences (P<0.05). The strain variable of meniscus posterior horn in 90° was significantly higher than that of other angles, and there was statistical difference (P<0.05). Conclusions Posterior cruciate ligament rupture can be treated with arthroscopy, which proves to be safe and effective.It can reduce the strain variable of meniscus posterior horn,which has benefit on knee joint.

Experiment research of cinobufacini on TGF-β1-induced epithelial-to-mesenchymal transition in human colorectal carcinoma cells / 重庆医学

Objective To investigate the effects of cinobufacini on TGF-β1-induced epithelial-to-mesenchymal transition of human colorectal carcinoma cells in vitro.Methods The cultured colorectal carcinoma cell line(SW480) was divided into the control group,TGF-β1 (10 ng/mL) individual treatment group and co-treatment groups with TGF-β1 (10 ng/mL) + cinobufacini (2.5,5,10,20,40,80 mg/mL),which were cultured in vitro for 48 h.The proliferation of the cells were measured by CCK8 assay.The morphological changes were observed by inverted phase contrast microscope.The ability of cell invasion and migration was detected by Transwell assay.The mRNA and protein expressions of E-cadherin and Vimentin were detected with QRT-PCR and Western Blot.Results (1)Compared with the TGF-β1 (10 ng/mL) individual treatment group,TGF-β1 (10 ng/mL) + cinobufacini (10,20,40,80 mg/mL) co-treatment groups significantly had significantly proliferation inhibitory effect on SW480 (P＜0.05).(2) Compared with the normal control group,TGF-β1 individual treatment group and TGF-β1 (10 ng/mL) + cinobufacini(2.5 mg/mL) group exhibited classical mesenchymal phenotype,while the TGF-β1 (10 ng/mL) + cinobufacini (5 mg/mL) co-treatment group showed classical epithelial phenotype.(3) The ability of invasion and migration in the TGF-β1(10 ng/mL)+ cinobufacini(2.5,5 mg/mL) co-treatment group were significantly weakened compared with the TGF-β1 individual treatment group (P＜0.01).(4) QRT-PCR and Western Blot results indicated that compared with the normal control group,the Vimentin expression in the in the TGF-β1 individual treatment group was significantly increased and the E-cadherin expression was significantly decreased.Furthermore,compared with the TGF-β1 individual treatment group and control group,the Vimentin expression level in the TGF-β1 (10 ng/mL) + cinobufacini (2.5,5 mg/mL) groups was significantly decreased and E-cadherin expression was significantly increased.Conclusion Cinobufacini can inhibit TGF-β1-induced cell proliferation in human colorectal carcinoma SW480 cells,and its mechanism may be related with promoting E-cadherin expression increase,meanwhile decreasing the vimentin expression,thus inhibiting the EMT process induced by TGF-β1.

Seroprevalence of Toxoplasma gondii Infection among HIV/AIDS Patients in Eastern China

Toxoplasmosis, a neglected tropical disease caused by the protozoan parasite Toxoplasma gondii, occurs throughout the world. Human T. gondii infection is asymptomatic in 80% of the population; however, the infection is life-threatening and causes substantial neurologic damage in immunocompromised patients such as HIV-infected persons. The major purpose of this study was to investigate the seroprevalence of T. gondii infection in subjects infected with HIV/AIDS in eastern China. Our findings showed 9.7% prevalence of anti-T. gondii IgG antibody in HIV/AIDS patients, which was higher than in intravenous drug users (2.2%) and healthy controls (4.7%), while no significant difference was observed in the seroprevalence of anti-Toxoplasma IgM antibody among all participants (P>0.05). Among all HIV/AIDS patients, 15 men (7.7%) and 10 women (15.9%) were positive for anti-T. gondii IgG antibody; however, no significant difference was detected in the seroprevalence of anti-Toxoplasma IgG antibody between males and females. The frequency of anti-Toxoplasma IgG antibody was 8.0%, 13.2%, 5.5%, and 0% in patients with normal immune function (CD4+ T-lymphocyte count ≥500 cells/ml), immunocompromised patients (cell count ≥200 and <500 cells/ml), severely immunocompromised patients (cell count ≥50 and <200 cells/ml), and advanced AIDS patients, respectively (cell count <50 cells/ml), while only 3 immunocompromised patients were positive for anti-T. gondii IgM antibody. The results indicate a high seroprevalence of T. gondii infection in HIV/AIDS patients in eastern China, and a preventive therapy for toxoplasmosis may be given to HIV/AIDS patients based on CD4+ T lymphocyte count.

Clinical Application of Multi-slice Helical CT Virtual Endoscopy in Colorectal Carcinoma / 实用放射学杂志

Objective To evaluate the methodology and the clinical usefulness of multi-slice helical CT virtual endoscopy(MSCTVE).Methods Twenty-six cases by suspected colorectal carcinoma underwent multi-slice helical CT virtual endoscopy after colon was cleaned and insufflated with air.The parameters were used:120 kV,250 mA,pitch 6,reconstructure thickness 2.5 mm,interval 1.0.All data sets were transferred to ADV 3.1 workstation,CTVE reconstruction were done with Navigator and used CTVE to view colorectal and rectum carcinoma.Of 26 cases,16 cases were proved by colonoscopy and 22 cases by operation.Results 14 cases carcinoma in rectum,8 cases in sigmoidcolon,1 case involved both rectum and sigmoidocolon.MSCTVE results were similar to colonography.Conclusion Multi-slice helical CT virtual endoscopy has clinical usefulness for detecting colorectal and rectum carcinoma as it is non-invasive,safe,feasible.MSCTVE is a supplement to colonoscopy especial in incomplete colonoscopy.

Multi-slice Helical CT Virtual Cholangioscopy:Preliminary ClinicalApplications in the Detection of Biliary Calculus / 实用放射学杂志

Objective To investigate the methods and clinical value of multi-slice CT virtual cholangioscopy in the detection of biliary calculus.Methods Volume scanning was performed in 38 patients with suspected biliary calculi after injection 50% Meglumine adipiodone injection 20 ml through vien.All data sets were transferred to ADV3.1 workstation.MSCTVC reconstruction were done with Navigator soft-ware.All cases were proved by ultrasound,axial CT and MSCT 3 D results or operations.Results MSCTVC could well demonstrate 3D fashion of biliary calculi and it was similar to ultrasound,axial CT and MSCT 3D results.Conclusion MSCTVC combined with CT 2D 3D results has a high diagnosis accuracy and clinical usefulness,thus,improve the diagnosis of biliary calculi.