Five new spirosterol saponins from Allii Macrostemonis Bulbus / 中国天然药物

Five new spirostanol saponins (1-5) and seven known compounds (6-12) were isolated from the n-butanol fraction of 75% ethanol extract of Allii Macrostemonis Bulbus. The identification and structural elucidation of all the isolates were performed through extensive 1D and 2D NMR experiments, HR-ESI-MS data analysis and comparisons with literature values. Antioxidant evaluation showed that compounds 6-11 exhibited certain scavenging effects on ABTS radical, where compounds 6, 7 and 11 had IC50 values of 0.208, 0.057 and 0.014 mg·mL-1, respectively.

Quality Comparison of Ziziphi Spinosae Semen under Different Packaging and Storage Conditions / 中国药房

ABSTRACT OBJECTIVE：To determine the suitable storage conditions，packaging materials and packaging methods of Ziziphi Spinosae Semen，and to provide reference for guaranteeing the quality. METHODS：The same batch of Ziziphi Spinosae Semen samples were packed in plastic woven bag，plastic bag，plastic bag in vacuum，aluminum-plastic composite bag，aluminum-plastic composite bag in vacuum and kraft coated paper bag. They were stored in a cool warehouse（temperature ≤20 ℃，relative humidity was 45％-75％） and drug stability test box [temperature was（40±2）℃，relative humidity was（75±5）％]. Five evaluation indexes were detected during 6-month of long-term cool stability test and accelerated stability test，including characters and the contents of moisture，aflatoxins，jujubosides A and spinosin. RESULTS：The results of 6-month long-term cool stability test showed that the character and the content of moisture，aflatoxins and jujubosides A of Ziziphi Spinosae Semen under different packaging conditions were in line with the requirements of 2015 edition of Chinese Pharmacopeia （part Ⅰ）（shorted for “pharmacopeia”）. The content of spinosin didn’t meet the requirement of pharmacopeia（no less than 0.080％），but the content of spinosin（0.079％）of aluminum-plastic composite bag in vacuum was close to the requirements of pharmacopeia. The results of 6-month accelerated stability test showed that the sample packed in kraft coated paper bag and plastic woven bag were seriously moldy；the sample packed in plastic bag and plastic bag in vacuum were blackened；the color of the sample packed in aluminum-plastic composite bag was slightly darker；but the appearance of the sample packed in aluminum-plastic composite bag in vacuum was basically unchanged. Only when stored for 2-month，the content of aflatoxin B1 in the sample packed in plastic woven bag was 8.64 μg/kg，which exceeded the pharmacopoeia regulation. The moisture content of the sample packed in plastic woven bag and kraft coated paper bag exceeded the highest value （9.0％） required by the pharmacopoeia. Although the content of jujuboside A of samples in each package decreased，it meet the requirements of the pharmacopoeia. Only the content of spinosin （0.084％）of sample packed in plastic bag in vacuum meet the requirements of the pharmacopoeia，and the content of spinosin （0.071％）of sample packed in aluminum-plastic composite bag in vacuum was relatively high. CONCLUSIONS：Ziziphi Spinosae Semen should be stored in a cool and dry place after packed in aluminum-plastic composite bag in vacuum.

Effect of Sodium Pyrosulfite Solution Soaking on Flavonoids in Astragali Radix / 中国药师

Objective: To analyze the contents of flavonoids in Astragali Radix before and after sodium pyrosulfite solution soa-king, so as to provide reliable methods for scientifically evaluating and effectively controlling the quality of Astragali Radix. Methods:After sodium pyrosulfite solution soaking, the contents of calycosin glucoside, ononin, formononetin and pterostilbene were analyzed by HPLC-DAD. Results: Calycosin glucoside, ononin, formononetin and pterostilbene showed a good linear relationship within the range of 0. 048-60. 000 μg·ml-1, 0. 019-30. 000 μg·ml-1, 0. 019-40. 000 μg·ml-1and 0. 019-40. 00 0μg·ml-1, respectively. After sodium pyrosulfite solution soaking, the contents of calycosin glucoside and ononin decreased, furthermore, with the increase of sodium sulfite solution concentration and soaking time, the decrease trend was more obvious, and the differences between the high concentra-tion group and the normal group were 2-3 times. The content of formononetin was essentially the same, while the difference in pterostil-bene content between the high concentration groups and the normal group was 10 times. Conclusion: After sodium pyrosulfite solution soaking, the contents of flavonoid glycoside components are reduced, therefore, it is not advisable to use sodium pyrosulfite solution soaking to achieve keeping fresh, mothproof and prolonging the shelf life of Astragali Radix.

Study on the Chemical Components in the Rattan of Rubia Argyi L / 中国药师

Objective:To investigate the chemical constituents in the rattan of Rubia argyi L.. Methods:The air-dried rattan of Rubia argyi L. was powdered and extracted three times by 75% ethanol with refluxing. After removing the solvent under the reduced pressure,the crude extract was dissolved in water,and then filtrated and extracted by petroleum ether and ethyl acetate to obtain crude extract after removing petroleum ether and ethyl acetate. The compounds were isolated and purified by silica gel column chromatogra-phy,reversed-phase silica gel column chromatography and Sephadex LH-20 gel column chromatography,and then identified based on physicochemical properties and spectral analysis(1 H-NMR and 13C-NMR). Results:Totally 13 compounds were isolated from the rat-tan of Rubia argyi L.,and characterized as secoisolariciresinol(1),xanthopurpurin(2),daucosterol(3),dehydroabietic acid(4), 2-hydroxy-1-methoxy-anthraquinone(5),β-sitosterol(6),lirioresinol A(7),2-hydroxy-7-methyl-9,10-anthraquinone(8),strych-novoline (9), ciwujiatone (10), 3,4-divanillyltetrahydrofuran (11), 2-(4-hydroxypheny) -6-(3-methoxy-4-hydroxyphenyl)-3,7-dioxabicyclo[3,3,0]octane (12), and (6S,9R)-vomifoliol (13).Conclusion: The compounds 1-13 are isolated from the rattan of Rubia argyi L. for the first time and the compounds 1,2,4,5 and 7-13 are first isolated from Rubia L..

UPLC Fingerprint of the Root of Rosa Laevigata Michx from Different Regions / 中国药师

Objective:To establish the fingerprint analysis method for the root of Rosa laevigata Michx from different regions by UPLC. Methods:The column was ACQUITY UPLC? Phenyl(2.1 mm × 100 mm,1.7 μm). The mobile phase consisted of methanol-water with gradient elution. The flow rate was 0. 2 ml·min-1 , the detection wavelength was 210 nm, the column temperature was 30℃, and the injection volume was 3 μl. Results:The fingerprint consisted of 15 common peaks. The range of similarity for twelve bat-ches of the root of R. laevigata Michx was 0. 489-0. 942. And the reference fingerprint of the root of R. laevigata Michx was estab-lished by UPLC. Conclusion:The fingerprint method is simple and reproducible, which can provide basis for the quality control and the medicinal resources exploration.

Chemical constituents of Dolomiaea souliei / 中国中药杂志

<p><b>OBJECTIVE</b>To study the chemical constituents of Dolomiaea souliei.</p><p><b>METHOD</b>Various chromatographic techniques were adopted to separate the constituents, and the spectrum analysis was made to identify their structures.</p><p><b>RESULT</b>Seventeen compounds were isolated and identified as: dehydrocostus lactone (1), costunolide (2), mokko lactone (3), santamarine(4), reynosin (5), 4alpha-hydroxy-4beta-methyldihydrocostol (6), sulfocostunolide A (7), beta-costic acid (8), beta-cyclocostunolide (9), vladinol A (10), ursolic acid (11), betulinic acid (12), betulin (13), dibutyl terephthalate (14), dibutyl phthalate (15), uridine (16), and emodin (17).</p><p><b>CONCLUSION</b>Compounds 6-9 and 12-17 were obtained from this genus for the first time, and compound 11 was obtained from this plant for the first time.</p>