Expression of heme oxygenase-1 in nasal polyps and regulation by glucocorticoid / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To evaluate the expression and possible modulation of heme oxygenase-1 (HO-1) in nasal polyps of patients with chronic rhinosinusitis with nasal polyps (CRSwNP).</p><p><b>METHODS</b>Nasal polyps and uncinate process tissues were collected from 25 CRSwNP patients and 19 healthy controls with nasal septal deviation. HO-1 expression was examined using qRT-PCR, immunohistochemistric staining and Western blot analysis. Moreover, additional uncinate process mucosal samples of 15 healthy controls with nasal septal deviation were harvested for nasal explant culture experiments. HO-1 expression was measured in cultured nasal explant in response to specific inflammatory and glucocorticoid stimulation. SPSS 20.0 software was used to analyze the data.</p><p><b>RESULTS</b>The mRNA and protein expression of HO-1 was significantly increased in polyp tissues, 1.220±0.397 in mRNA and 1.409±0.701 in protein, compared with healthy controls 0.464±0.318 in mRNA and 0.017±0.1147 in protein (U=22.00 in mRNA and U=1.00 in protein, both P< 0.05). The immunohistochemical results showed that HO-1 was mainly distributed in the epithelial layer, submucosal glands and inflammatory cells in nasal tissues. Nasal explant culture experiments demonstrated that HO-1 mRNA was upregulated by IL-17A. The HO-1 mRNA level before the stimulation was 1.000, and 17.264±4.275 after the stimulation of 1 ng/ml IL-17A (U=0, P<0.05), 19.128±4.605 after the stimulation of 10 ng/ml IL-17A (U=0, P<0.05), but was significantly suppressed after stimulation with glucocorticoids (dexamethasone, DEX). The mRNA level after the glucocorticoids stimulation was 0.370±0.101 (U=0, P<0.05) and 0.316±0.167 (U=0, P<0.05) respectively. Furthermore, the HO-1 mRNA was inhibited by TGF-β1, the mRNA level was 0.217±0.322 (U=0, P<0.05), 0.070±0.070 (U=0, P<0.05), respectively.</p><p><b>CONCLUSION</b>Increased HO-1 expression may play a role in the pathogenesis of CRSwNP, which may be considered as the therapeutic target.</p>

Application of computer assisted navigation system in endoscopic sinus and skull base surgery / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the applicative value of computer assisted navigation system in endoscopic sinus and skull base surgery.@*METHOD@#Forty-nine endoscopic surgery procedures were performed with the help of computer assisted navigation system, among which there were 25 cases of recurrent sinusitis and nasal polyps, 9 cases of nasal and sinus tumour, 7 cases of cerebrospinal fluid rhinorrhea, 2 cases of meningoencephalocele, 4 cases of congenital choanal atresia, 1 case of pituitary tumor and 1 case of foreign body in middle cranial fossa.@*RESULT@#The preoperative time was 5-13 minutes for preparation, 7 minutes in average. The target error was less than or equal to 1.5 mm. All the 49 cases had successful surgery without complications.@*CONCLUSION@#Computer assisted navigation system can help the surgeon to determine the sinus, skull base and adjacent anatomic landmarks correctly, improve surgical accuracy and safety, and reduce intraoperative and postoperative complications.

Application value of dual-source CT in the diagnosis of suspicious airway foreign bodies in children / 临床耳鼻咽喉头颈外科杂志

OBJECTIVE@#To evaluate the clinical value of dual-source computer tomography in the diagnosis of suspicious airway foreign bodies in children.@*METHOD@#Twenty-two children of suspicious airway foreign bodies were examined by DSCT. The original scanning data were reconstructed with MPR, MinP, CTVE and VR. The results were compared with surgical findings.@*RESULT@#The position, size, shape and accompanying signs of foreign bodies were clearly demonstrated by DSCT. Twenty cases were diagnosed as foreign bodies in the airway by DSCT. Nineteen cases were confirmed as foreign bodies. Purulent discharge was found by bronchoscopy in 1 case. The rate of conformed diagnosis was 95%.@*CONCLUSION@#DSCT can demonstrate the foreign body clearly and play an important role in the diagnosis of suspicious airway foreign body in children.