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BACKGROUND: Fracture healing occurrs by two ways: healing by first intention and healing by second intention, with the latter one similar to natural healing process. It has been proved that only 37% tibial farcture can reach healing by first intention, even it is fixed with strong AO steel plate. Different materials for intramedullary fixation have different influences on callus formation and callus content in the process of fracture healing.OBJECTIVE: To explore the influences of different internal fixations on callus formation and content in the course of fracture healing analysis with X ray computer image.DESIGN: Randomized controlled trialSETTING: At the Department of Orthopeadics of Changhai Hospital Affiliated to the Second Millitary Medical University of Chinese PLA and the Experimental Animal Center of the Second Millitary Medical University of Chinese PLA.MATERIALS: This study was carried out at the Experimental Animal Center and Orthopeadic Laboratory of the Second Millitary Medical University of Chinese PLA between March 2000 and July 2001. Totally 51New Zealand rabbits were randomly selected without gender limitation and divided, accccording to intermeduallry nails, into three groups with 17 rabbits in each group: rectangle-shaped intramedullary nails (RIN), Ender's nails (END) and stainless steel plates (SPL) groupsMETHODS: Fracture models were succccesufly established on rabbits.And then intramedullary nails were fixed 2 mm below the border of left tibial and fibula without exopexy. Positve and lateral X-ray films of each operational tibia were obtained at postoperational 2,3,4,8,12 weeks.Three rabbits in each group were killed for callus observation with naked eyes, and the maximal callus diameter at different time points was measured. The X-ray films at different periods were read carefully and quanatatively analyzed with computor image analysis system (lAS). The difference was compared with the analysis of variance and SNK test.MAIN OUTCOME MEASURES: The maximal callus diameter was determined with X-ray image at different time points so as to calculate the corrssponding integral of callus grey level.RESULTS: Totally 51 rabbits were enrolled in this study, 6 missed the experiment because 4 died for diarrhea and other two has exposed bone due to local infection, and then two rabbits were derived from each group; thereby, data of 45 rabbits were subjecte to the final statistical to the epak at postoperational 8 weeks; it was the longest in RIN group of the same stage,followed by Ender group and SPL group [(11.24±0.38), (10.86±0.65), (8.12±0.36) mm at postoperatively 4 weeks; ( 13.56±0.88 ), (12.84±0.20), (10.52±0.68) mm at postoperatively 8 weeks; ( 12.66±0.65 ), ( 11.84±0.55 ), (9.68±0.27) mm at postoperatively 12 weeks]. The difference between RIN, Ender and SPL was statistically significant, but the difference between the former two was unobvigroup; at the same postoperational time point, the intergral of callus grey level was the highest in RIN group, followed by Ender group and SPL group (89.11 ± 1.05,86.42±3.12,47.28 ± 1.57 at postoperatively 4weeks;159.69 ±3.64,148.72 ±1.68,79.63 ±2.41 at postoperatively 8 weeks;192.46 ±4.96,186.53 ±1.84,107.34 ±2.37 at postoperatively 12 weeks),The difference between RIN, Ender and SPL was obvious and moreover,the difference between RIN and Ender was significant at 8th week.CONCLUSION: The callus content in RIN and Ender group were higher than that in SPL group at each postoperational time point, which benefited for fracture healing. Callus content examination is a traditional means for the judgement of fracture healing. It prossesses that properties of intuition and persicuity, reliability, simplicity and practicibility. Moreover, subjectivity could be avioided if used in combination with X-ray image analysis system, since the digital management can help judging not only callus content but also callus quality, which may provide guidance for clinical treatment.
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1 kHz at least; For the cerebral infarction cases with carotid atheromatous plaque, the frequence plots for f max , f mode and f mean were separated throughout the cardiac cycle, f max in systole
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This study is to set up a method for separating,culturing and identifying neural stem cells from cortex in rat embryo,observing their characteristics of multipotentiality and differentiation in vitro. By using serum-free cell culturing and clone technology,the stem cells were isolated,cultured and passaged from cortex in 14-day old rat embryo. The expression of Nestin antigen of brain cells were investigated by immunocytochemistry and H-E staining. It proved that cells separated from cortex in rat embryo possessed the potential to form clones and express nestin special antigen,the differentiated cells could express special antigen of neurons,astrocytes,and oligodendrocytes,they were stem cells of central nervous system.
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For developing Digital Image Expert System in rhinoplasty, the accurate and reliable Digital Image Measuring System must be first set up, and the data from the Digital Image Measuring System must be reliable with the data measured by hand. The measuring system mainly consists of a computer with an image processing board, a TV camera and a head fixing device. Ten adults were selected randomly and some indexes of head and face were measured with the measuring system and by hand respectively. The reliability of the measuring system was confirmed by the statistical analysis of data measured. Finally, the results were also discussed.
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Four anthropometric-measurements (weight, height, head ciroumference and chest circumference) of 38342 children aged from birth to 12 years in PLA were made. The results showed that the growth and development of military children conform to the general laws of physicl development of children. The mean weight, head ciroumference and chest ciroumference by age of children aged from birth to 7 years in PLA were higher than that in the nine cities.
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Single factor analysis of intelligence test of children aged from birth to 12 years in PLA was made. Results showed that the dangerous factors in mental development included twins, low birth weight neonate, premature neonate, severe asphyxia, encephalitis and medium or severe cranium trauma. On the other hand, many factors (single child in family, comfortable family, better educated raiser and the mother's child-bearing age from 25 to 35 years) could promote mental development. It is indicated that children mental health care were closely related to birth control, perinatal health care and improving economical and culture quality of our country.
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Scatchard, pseudoscatchard and competitive analyses showed that there were two glucocorticoid (GC) binding sites in rat hepatic cytosol: high-affinity glucocorticoid binding sites (HAGS) and low-affinity glucocorticoid binding sites (LAGS). Pseudoscatchard analysis showed the dissociation constant (Kd) of LAGS was 4.1+1.4umol/L (n=4). The inactivation of LAGS in vitro was slower" than that of HAGS. LAGS and HAGS could not be separated by DEAE-cellulose chfomatography. HAGS were saturable and had steroid specificity for glucocorticoid just as the classic glucocorticoid receptor. Triamcinolone acetonide, RU486 and RU26988 competed equally well to LAGS while aldosterone, progesterone, estradiol and testosterone did not compete with LAGS. These results suggest that LAGS have steroid specificity for glucocorticoids. The biological actions of LAGS await fur ther study.