RESUMEN
Effect of genistein, a protein tyrosine kinase inhibitor, on TAME-esterase induced contractions were studied on rat aorta strips in vitro. Data showed that TAME-esterase induced contractions were concentration dependent and these contractions were significantly inhibited when rat aorta strips were pre-incubated with genistein. The present findings provide evidence for the possible contribution of tyrosine kinases during TAME-esterase induced contractions in aorta.
Asunto(s)
Animales , Aorta/efectos de los fármacos , Inhibidores Enzimáticos/farmacología , Genisteína/farmacología , Masculino , Contracción Muscular/efectos de los fármacos , Péptido Hidrolasas/fisiología , Proteínas Tirosina Quinasas/antagonistas & inhibidores , Ratas , Ratas Sprague-DawleyRESUMEN
Contractions induced by TAME-esterase on rat aorta strips mounted in vitro were significantly inhibited in presence of Vitamin C. The work lends support to the role of ascorbic acid in preventing endothelial dysfunction through release of nitric oxide. It is suggested that conclusions TAME-esterase could be an important biological marker associated with onset of vascular discases such as hypertension.
Asunto(s)
Animales , Aorta Torácica/efectos de los fármacos , Ácido Ascórbico/farmacología , Endotelio Vascular/efectos de los fármacos , Contracción Muscular/efectos de los fármacos , Péptido Hidrolasas/farmacología , Ratas , Ratas Sprague-DawleyRESUMEN
Possible pharmacological effects of N-alpha-tosyl L-arginine methyl ester [TAME] were studied on rat aorta strips in vitro. Results showed that [TAME]-esterase was an endothelium dependent component that involved a nitric oxide cyclic-GMP mediated pathway. Furthermore, during activation of Kinin-Kallikrein system, TAME-esterase induced contractions involve degradation of kinins by kininases.