RESUMEN
A modified has been used for the determination of PSA by enzyme- linkedimmunosorbent assay [ELISA], in which commercial kits are used, that is nowavailable in the market instead of preparing the reagents. Normal, oligo andazoospermic seminal samples were collected with other body fluids [blood,urine, milk, saliva, vaginal secretions and 12 hours post-coital vaginalswabs]. The results revealed that PSA is a useful semen market, even in casesof azoospermia, and can be also detected in the vaginal swabs that have beentaken 12 hours after coitus. No true cross reactions were found between theanti-PSA and other body fluids, including vaginal secretions. This method isvery sensitive and can detect semen in very small amount [0.5 mu l]. The fourdifferent studied semen markets could be measured easily, even after two months. The higher concentrations of these markets were obtained in stains that were kept refrigerated in comparison with other strong temperature, this was observed at all storage periods. Statistically significant differences have been recorded between fertile and infertile groups only for GGt and LDH but neither for PSA nor PSA not PAP. The best conserved activities after 2 months were those of GGT followed, then LDH and lastly PAP