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1.
Iranian Journal of Veterinary Research. 2016; 17 (1): 8-12
en Inglés | IMEMR | ID: emr-185341

RESUMEN

Foot-and-mouth disease is an important viral disease of cloven-hoofed animals. Inactivated whole particle virus vaccines are still widely used in prophylactic vaccination campaigns. The choice of adjuvant is a very important factor in enhancing immune responses and the efficacy of inactivated vaccines. Montanide ISA 61 VG is a new ready-to-use mineral oil-based adjuvant developed by SEPPIC Inc. [SEPPIC, France] with high-potential immune responses needed for clinical protection against FMD infection. In this study, we compared the efficacy of two FMD vaccines either formulated with the new oil-based adjuvant ISA 61 VG and saponin, or with aluminum hydroxide gel and saponin. Both vaccines contained the same antigen payloads of O2010/IR. Two groups of 15 naive cattle received a single vaccination with different doses [full dose, 1/3 dose and 1/9 dose] to calculate their PD50 [50% protective dose] after being challenged with the homologous virulent virus. The mean neutralizing antibody titer was determined at 0, 7, 14 and 21 days after vaccination, measured by a micro neutralization test. The new vaccine improved humoral immune responses by 19%, while inducing a higher geometric mean. The titer for neutralizing antibodies was 2.91 log10 compared to the alum-gel based adjuvant vaccine which was 2.44 log10 [P-value=0.1782]. The new vaccine showed a PD50 value of 10.05 as compared to a PD50 value of 4.171, respectively. According to the results, the FMD vaccine formulated with the new oil adjuvant, ISA 61 VG, shows potential as an alternative vaccine for routine and emergency vaccinations in the FMD enzootic region

2.
Journal of Veterinary Research. 2009; 64 (2): 141-146
en Persa | IMEMR | ID: emr-134565

RESUMEN

During 1387-86, total number of 120 blood samples gathered from sheep with bluetongue-like clinical sign. The samples collected from seropositive regions .After separating serum, they were evaluated by competitive ELISA for detecting Ab against VP7 antigen. The full length of S7 gene [1156 bp] amplified by one step RT-PCR .In this method two sets specific primers, targeting 3? and 5? ends of S7 segment, were applied. For confirmation of PCR products in first amplification, nested-PCR was used. By using internal primers the most samples which displayed weak S7 band, produced a sharp and specific internal band [769 bp].By this method the sensitivity of virus detection dramatically increased .Among the blood samples, the number of BTV serogroup positive, nPCR positive, both BTV serogroup and nPCR positive and both BTV serogroup and nPCR negative cases were determined, 41 [34.8%], 23 [19.2%], 12 [10%] and 56 [46.6%] respectively. This is the first report about using RT-PCR for BTV detection in Iran. RT-PCR and nPCR molecular technique can be used as a very sensitive and reliable method for BTV detection in blood samples


Asunto(s)
Animales , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Lengua Azul , Ensayo de Inmunoadsorción Enzimática , Reacción en Cadena de la Polimerasa
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