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1.
IRCMJ-Iranian Red Crescent Medical Journal. 2009; 11 (3): 286-294
en Inglés | IMEMR | ID: emr-94025

RESUMEN

One of the valuable tools for inhibiting the specific gene expression is antisense technique. To determine T cell responses, co-stimulatory molecule expression on the antigen presenting cells is important. In the present study, the effects of high affinity antisense against CD40 mRNA on the function and phenotype of DCs [dendritic cells] were investigated. The DCs were separated from the mice spleens and then cultured in vitro. By means of lipofectamine 2000, the antisense was delivered into the cells and the efficacy of transfection was estimated by flow cytometry. Also, the mRNA expression and protein synthesis were assessed by real time PCR and flow cytometry, respectively. The DCs were transfected with 6 M antisense and 2 l lipofectamine 2000. The percentage of CD40 expression in DCs was 38%. The results showed that CD40 expression is reduced in DCs to 22% and 24%. By annexine V and propidium iodine staining, we could evaluate the viability of the transfected cells. The inhibition of CD40 gene expression was associated with the increase in IL-4 secretion. This shifted the DCs to stimulate Th2 cytokine production from the allogenic T cells. In addition, in the MLR, the DCs without CD40 expression showed poor allostimulatory effects. This finding is valuable in the study of the costimulatory molecules of DCs. These data demonstrate that direct interference of the cell surface expression of CD40 at transcriptional level by antisense confers tolerogenecity potential of DCs. This approach is a useful tool through which DCs become tolerogenic and can be studied as a potential therapeutic option for the autoimmune diseases and allograft rejection


Asunto(s)
Masculino , Animales de Laboratorio , Antígenos CD40 , Ratones Endogámicos BALB C , ARN Mensajero , Fenotipo
2.
JBUMS-Journal of Babol University of Medical Sciences. 2005; 7 (3): 46-49
en Persa | IMEMR | ID: emr-168769

RESUMEN

Trichomoniasis is an infection of women urogenital tract that is transferred almost in all cases by sexual contact. This study was done to compare clinical presentations of trichomoniasis and several different paraclinical methods to determine simplest and most efficient method in doubtful patients to trichomonas infection in order to treat infected women and prevent from immethodical use of antibiotics and creation resistant strains. One hundred and fifty fertile women [15-50 years aged] that attended in gynecological clinics and had clinical pictures of trichomoniasis were studied to assess for the presence of trichomonas vaginalis by history, wet mount, papanicolaou smear and dorset's culture. Then data were analyzed. In 150 doubtful patients to trichomoniasis, average of age was 30.56 years. The most common symptoms were frequent pain with sexual intercourse and itching in genital area after persistent, malodor, frothy and yellow vaginal discharge. The most common sign in pelvic examination were frothy, yellowish discharge accompanied by cervical erosion. In 28 patients [18.67%] wet smear, Pap smear and culture were positive simultaneously, papanicolaou smear was positive only in 38 patients. Wet smear is most sensitive and specified for diagnosis of trichomonas vaginalis in doubtful female patients to trichomoniasis, and because of over diagnosing according to clinical manifestation, a confirmatory test should be performed before initiating therapy

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