RESUMEN
Aims: To investigate the cytotoxic properties of different polarity solvents of Polygonum minusextracts towards colon cancer cell lines, HT-29, HCT-116 and CT-26.Study Design:Experimental study. Place andDuration of Study:Central Laboratory, Tissue Culture Laboratory, Universiti Sultan Zainal Abidin, Terengganu between September 2019 until December 2019.Methodology:The different polarity solvents of P. minusextracts had been led to tetrazolium salt reduction (MTT) assay and an inhibition concentration of 50 (IC50) value for their cytotoxic potential against colon cancer cells. Then, cell morphology observation and fluorescence double staining of treatment cells were determined using a light inverted microscope and acridine orange/propidium iodide staining.Results:The results indicated that an extraction yields aligned from 0.01% for acetone and ethyl acetate to 0.45% for aqueous solution with decreasing order of aqueous solution > 70% aqueous ethanol > 50% aqueous ethanol > methanol > ethanol > acetone and ethyl acetate. Meanwhile, the ethyl acetate extract showed a higher cytotoxic effect at IC50values of 7.00 ± 0.06 μg/mL and 7.00 ± 0.30 μg/mL towards the HCT-116 and CT-26 cells; and 50% aqueous ethanol towards HT-29 cells (24.00 ± 0.01 μg/mL). The different solvent extracts of P. minusinduced cytotoxic effects on the treated cell lines by altering their normal cell morphology and cell membrane integrity (except for acetone extract). Conclusion:Therefore, the use of different polarity solvent extracts of P. minusas an anti-cancer agent is promising more on ethyl acetate and warrants further investigation
RESUMEN
Aims: To examine the anti-proliferative properties of different extracts of new variety an organic rice MRQ74 towards colon cancer cells: in-vitrostudy.Study Design:Experimental study. Place and Duration of Study:Central Laboratory, Tissue Culture Laboratory, University of Sultan Zainal Abidin, Terengganu from November 2019 until February 2020.Methodology:The organic rice MRQ74 extracts had been led to tetrazolium salt reduction (MTT) assay and an inhibition concentration of 50 (IC50) value for their cytotoxic potential against colon cancer cells. Meanwhile, cells morphology observation and fluorescence double staining of treatment cells were determined using a light inverted microscope and acridine orange/propidium iodide staining.Results:Results showed that 50% aqueous ethanol of rice grains gave the lowest IC50values towards HCT-116 and CT-26 cell lines, while an aqueous solution of rice grains gave the lowest C50values towards HT-29 cells (p<0.05). Thoroughly, the treated colon cancer cells had shown morphological alterations after treated with different solvent extracts of an organic rice MRQ74.Conclusion:The outcomes had observed preliminary results on cancer study for better health by inspiring the consumption of an organic rice MRQ74 and future product development