RESUMEN
OBJECTIVE@#To study the role of dopamine D3 receptor involved in the amphetamine-induced conditioned place preference (CPP) in mice.@*METHODS@#The CPP was observed in D3 receptor knock-out (D3RKO) mice and C57BL/6 wild-type control mice after administration of amphetamine. The data were analyzed with a two-way ANOVA using the SPSS 13.0 software.@*RESULTS@#D3RKO mice showed a significant amphetamine-induced CPP (P<0.001), compared with the ones administered with saline in C57BL/6 control mice.@*CONCLUSION@#The results indicate that amphetamine can produce significant CPP in dopamine D3 receptor knock-out mice, suggesting that amphetamine-induced addiction can be inhibited by dopamine D3 receptor.
Asunto(s)
Animales , Femenino , Masculino , Ratones , Anfetamina/farmacología , Conducta Animal/efectos de los fármacos , Condicionamiento Operante/efectos de los fármacos , Modelos Animales de Enfermedad , Técnicas de Inactivación de Genes , Ratones Endogámicos C57BL , Ratones Noqueados , Actividad Motora/efectos de los fármacos , Tiempo de Reacción , Receptores de Dopamina D3/fisiologíaRESUMEN
<p><b>OBJECTIVE</b>To study the short teadem repeat(STR) genetics structure of a Chinese Yunnan Yi racial group.</p><p><b>METHODS</b>Genetic distributions for nine STR loci were determined based on STR gene scan marked by fluorescence.</p><p><b>RESULTS</b>Sixty-nine alleles and 164 kinds of genotypes were detected and identified from 84 unrelated Yi racial individuals. The corresponding gene and genotype frequencies were in 0.0060-0.5060 or 0.0119-0.4167 respectively. The expected and observed genotype frequencies of nine STR loci were in accordance with the Hardy-Weinberg equilibrium(P>0.05). The statistical analyses of nine STR loci showed that PIC was distributed in 0.5804-0.8777, H was in 0.6507-0.8002, DP was in 0.7976-0.9558, EPP was in 0.5207-0.8386, except TPOX and THO1 loci.</p><p><b>CONCLUSION</b>Above research data enrich the Chinese genetic database, and play an important role in Chinese genetic study and in forensic application.</p>