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Objective To investigate the relationship between the expression of leptin protein and clinicopathological features and prognosis of gastric cancer. Methods The cancer tissue specimens of 112 patients diagnosed with gastric cancer in Shaanxi Provincial Cancer Hospital from January 2009 to December 2012 were collected. Random number table method was used to select 50 cases of paracancerous normal mucosa tissues as the controls. The expression of leptin protein was detected by using immunohistochemistry. And the relationship between the expression of lepin protein and the clinicopathological features and prognosis was analyzed. Results The positive rate of leptin protein in gastric cancer tissues was higher than that in paracancerous normal gastric mucosa tissues, and the difference was statistically significant [68.8％ (77/112) vs. 34.0％ (17/50), χ2= 17.139, P< 0.01]. The expression of leptin protein was associated with tumor vessel invasion, invasion depth and TNM stage (all P< 0.05). The expression of leptin protein was positively correlated with tumor invasion depth and TNM stage (r=0.265, P=0.005;r=0.318, P=0.001). The 5-year total survival rates of patients with positive and negative expression of leptin protein were 65.2％ and 78.4％, respectively, and the difference was statistically significant (χ2= 9.526, P= 0.002). The 5-year total survival rates of patients who received neoadjuvant chemotherapy or not were 71.7％ and 67.6％, respectively, and the difference was not statistically significant (χ2= 2.321, P= 0.128). In patients with positive expression of leptin protein, 5-year total survival rates of patients who received neoadjuvant chemotherapy or not were 47.4％ and 74.2％ , respectively (χ2= 6.336, P= 0.012). In patients with negative expression of leptin protein, 5-year total survival rates of patients who received neoadjuvant chemotherapy or not were 92.3％and 18.2％, respectively (χ2 = 14.237, P< 0.01). Cox multivariate regression analysis showed that leptin protein expression, lymph node metastasis, pathological grading, vessel invasion and TNM stage were independent prognostic factors for patients with gastric cancer (all P < 0.05). Conclusion Leptin protein is highly expressed in gastric cancer tissues, and it can be used as a marker for judging the prognosis and chemotherapy effect of gastric cancer patients.
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Objective@#To investigate the relationship between the expression of leptin protein and clinicopathological features and prognosis of gastric cancer.@*Methods@#The cancer tissue specimens of 112 patients diagnosed with gastric cancer in Shaanxi Provincial Cancer Hospital from January 2009 to December 2012 were collected. Random number table method was used to select 50 cases of paracancerous normal mucosa tissues as the controls. The expression of leptin protein was detected by using immunohistochemistry. And the relationship between the expression of lepin protein and the clinicopathological features and prognosis was analyzed.@*Results@#The positive rate of leptin protein in gastric cancer tissues was higher than that in paracancerous normal gastric mucosa tissues, and the difference was statistically significant [68.8% (77/112) vs. 34.0% (17/50), χ2 = 17.139, P < 0.01]. The expression of leptin protein was associated with tumor vessel invasion, invasion depth and TNM stage (all P < 0.05). The expression of leptin protein was positively correlated with tumor invasion depth and TNM stage (r = 0.265, P = 0.005; r = 0.318, P = 0.001). The 5-year total survival rates of patients with positive and negative expression of leptin protein were 65.2% and 78.4%, respectively, and the difference was statistically significant (χ 2 = 9.526, P = 0.002). The 5-year total survival rates of patients who received neoadjuvant chemotherapy or not were 71.7% and 67.6%, respectively, and the difference was not statistically significant (χ 2 = 2.321, P = 0.128). In patients with positive expression of leptin protein, 5-year total survival rates of patients who received neoadjuvant chemotherapy or not were 47.4% and 74.2%, respectively (χ 2 = 6.336, P = 0.012). In patients with negative expression of leptin protein, 5-year total survival rates of patients who received neoadjuvant chemotherapy or not were 92.3% and 18.2%, respectively (χ 2 = 14.237, P < 0.01). Cox multivariate regression analysis showed that leptin protein expression, lymph node metastasis, pathological grading, vessel invasion and TNM stage were independent prognostic factors for patients with gastric cancer (all P < 0.05).@*Conclusion@#Leptin protein is highly expressed in gastric cancer tissues, and it can be used as a marker for judging the prognosis and chemotherapy effect of gastric cancer patients.
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Objective To investigate the relationship between vascular endothelial growth factor C (VEGF-C) and lymphatic vessel density (LVD) with clinicopathological features of the patients in stage Ⅱ colorectal cancer.Methods Fifty-seven tissues in stage Ⅱ colorectal cancer and 57 normal mucosal tissues in Shaanxi Provincial Cancer Hospital from January 2005 to December 2010 were collected.The expression of VEGF-C and LVD were detected by using immunohistochemical method.The relationship between VEGF-C and LVD with the clinicopathological features of colorectal cancer was analyzed.Results The expression of VEGF-C and LVD in cancer tissues was higher than that in normal tissues (6.80±1.24 vs.2.07±0.17,t =3.773,P =0.000;3.19±0.53 vs.1.84±0.26,t =2.276,P =0.025).There were statistical differences between VEGF-C and LVD in different tumor diameter (4.00±0.54 vs.11.26±2.89,t =-3.049,P =0.004;1.89±0.37 vs.5.27±1.12,t =-3.376,P =0.001) and relapse or metastasis (4.74±1.31 vs.10.62 ±2.39,t =-2.158,P =0.039;1.86±0.45 vs.5.65±1.06,t =-3.778,P =0.000).LVD in VEGF-C positive group was higher than that in VEGF-C negative group (4.16±0.64 vs.0.21±0.11,t =3.503,P =0.001).The expression of VEGF-C was positively correlated with LVD (r =0.892,P =0.000).Conclusions The expression of VEGF-C in stage Ⅱ colorectal cancer is correlated with LVD,as well as tumor size and relapse or metastasis.VEGF-C and LVD could also play important roles in the occurrence,development and metastasis in stage Ⅱ colorectal cancer.
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Objective To investigate the expressions and significances of phosphatase and tension homologous genes (PTEN) and human telomerase reverse transcriptase (hTERT) in esophageal cancer.Methods 84 specimens of esophageal cancer underwent surgical resection from December 2010 to December 2012 were collected,at the same time,30 cases of para cancerous tissues and 30 normal esophageal mucosa tissues were taken as control.Streptavidin peroxidase (SP) immune-histochemical method was used to detect the expressions of PTEN and hTERT in the esophageal tissue specimens,according to the clinical data,the relationships between PTEN,hTERT in the esophageal cancer tissues with clinical pathological features and prognosis was analyzed.Results (1) The positive expression rate of PTEN in esophageal cancer tissue was significantly lower than that in para cancerous tissue (x2 =5.077,P =0.024) and normal mucosa tissue (x2 =22.810,P =0.000);the positive expression rate of PTEN in para cancer tissues was significantly lower than that in normal mucosa (x2 =5.104,P =0.024).(2) The positive expression rate of hTERT in esophageal cancer tissues was significantly higher than that in para cancerous tissue (x2 =19.724,P =0.000) and normal mucosa tissue (x2 =46.392,P =0.000);The positive expression rate of hTERT in para cancerous tissues was significantly higher than that in normal mucosa (x2 =4.565,P =0.033).(3) The expression intensities of PTEN and hTERT in esophageal cancer tissues were related to the degree of tumor invasion,clinical stage,lymph node metastasis and degree of differentiation (P ＜ 0.05).(4) At the end of the follow-up,the median survival period of all patients was 22 months,the cumulative survival rates of 1,3,and 5 years after operation were 65.48％,38.10％ and 28.57％ respectively.The cumulative survival rates of 1,3,and 5 years after operation in patients with low expression of PTEN and patients with high expression of PTEN were 60.00％ and 84.21％,30.77％ and 63.16％,23.08％ and 47.37％,respectively.The survival rate of patients with high expression of PTEN was significantly higher than that of patients with low expression of PTEN (x2 =5.073,P =0.024).The cumulative survival rates of 1,3,and 5 years after operation in patients with low expression of hTERT and patients with high expression of hTERT were 76.47％ and 58.00％,50.00％ and 30.00％,38.24％ and 22.00％,respectively.The survival rate of patients with high expression of hTERT was significantly lower than that of patients with low expression of hTERT (x2 =4.174,P =0.041).Conclusions The expression of PTEN was low in esophageal cancer tissues and high in hTERT,which could be used as a predictor of clinical prognosis in patients with esophageal cancer.
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Objective To study the relationship between expression of leptin and clinical pathogenesis and prognosis of stage Ⅱ colorectal cancer.Methods The clinical data of 102 patients with colorectal cancer admitted to Shaanxi Provincial Tumor Hospital between January 2005 and December 2010 was collected and statistically analyzed.The expression of leptin in postoperative colorectal cancer tissues and 40 normal colorectal tissues was detected by P-V immunohistochemical technique.Results The expression of leptin in cancer tissues were obviously higher than that in normal tissues [59.8 ％ (61/102) vs.17.5 ％ (7/40),x 2 =20.605,P =0.000].There was no association between leptin expression level and gender,age,tumor location,pathological grading,pathological types,tumor size,invasion depth and body mass index (all P ＜ 0.05).Cox multivariate regression analysis showed that leptin expression (P =0.041) and adjuvant chemotherapy (P =0.008) were independent factors affecting OS in patients with stage Ⅱ colorectal cancer.The patients with positive expression of leptin could not benefit from the adjuvant chemotherapy (P =0.259),and patients without leptin expression had improved survival when treated by adjuvant chemotherapy (P =0.021).Conclusion The expression of leptin in stage Ⅱ colorectal cancer tissues is obviously higher than that in normal tissues.Leptin is associated with chemotherapy resistance and therapy-independent prognosis.