Inhibiting miR-186 expression alleviates mitochondrial damage in hypoxic human umbilical vein endothelial cells / 南方医科大学学报

OBJECTIVE@#To investigate the effect of miR-186 inhibition on the expression of hypoxia-inducible factor-1α (HIF-α) and mitochondrial function in hypoxic vascular endothelial cells.@*METHODS@#Human umbilical vein endothelial cells (HUVECs) cultured in routine or hypoxic conditions for 6 h were examined for the expression of miR-186. A miR-186 inhibitor was transfected in the HUVECs, and the cells were subsequently cultured in hypoxic condition for 6 h to observe the changes in the mitochondrial structure under an electron microscope. The changes in the mRNA and protein expressions of HIF-1α in response to miR-186 interference were tested using real-time fluorescent quantitative PCR and Western blotting.@*RESULTS@#The expression of miR-18 was mildly increased in HUVECs after hypoxic exposure for 6 h (=0.0188). Interference of miR-186 expression obviously promoted the mRNA and protein expressions of HIF-1α in HUVECs. In hypoxic conditions, miR-186 interference significantly reduced mitochondrial damage in HUVECs as observed under electron microscope (=0.0297).@*CONCLUSIONS@#Inhibition of miR-186 protects vascular endothelial cells against hypoxic injuries by promoting HIF-α expression to lessen mitochondrial damage, suggesting the possibility of targeted miR-186 interference for treatment of hemorrhagic shock.

老年性前列腺增生伴糖尿病的外科治疗及护理 / 中国康复理论与实践

@#目的探讨对老年性前列腺增生症合并糖尿病患者手术方法的选择和围手术期的护理。方法对49例老年性前列腺增生症合并糖尿病患者行经尿道前列腺汽化电切术(TURVP) 30例、耻骨后前列腺摘除术19例;连续监测血糖,适当调整胰岛素用量,将血糖控制在空腹<7.1mmol/L、餐后<13mmol/L;残余尿超过80ml则留置尿管引流;口服抗生素预防感染。结果行TURVP者术后6—24h尿液即清亮;行开放性手术者均采用持续膀胱冲洗;全部切口Ⅰ期愈合;血糖控制在正常水平。结论对伴有糖尿病的老年性前列腺增生症患者,选择合适的手术方式与相应的围手术期护理是手术成功的关键。