Application of xenotransplantation in clinical practice / 器官移植

Organ transplantation is the optimal treatment for end-stage organ failure. Nevertheless, organ shortage is a global problem, which limits further development of organ transplantation. Recent research shows that genetically modified pig may become a realistic alternative source of clinical organ transplantation donor. Xenotransplantation may serve as one of the effective measures to resolve the problem of organ shortage. Since 2021, 2 cases of living xenotransplantation and 6 cases of xenotransplantation in brain death recipients have been performed worldwide, and phase Ⅰ clinical trial of xenotransplantation has been launched, and the results have exceeded expectations. Therefore, in this article, recent clinical trial results of xenotransplantation in living and brain death recipients were retrospectively analyzed, and scientific, technical and ethical issues related to clinical research of xenotransplantation were illustrated, hoping to provide reference for clinical research of xenotransplantation in China and promote the development of xenotransplantation in clinical practice.

Effects of fragmented sleep on motor behavior and mental health among school-age adolescents / 中国学校卫生

Abstract@#As a new social phenomenon, fragmented sleep breaks through the limitations of time and space, and provides self comfort for teenagers to learn entertainment at night, but the moderation of fragmented sleep must be studied. The paper ellucidates the essence of fragmented sleep, probes into the reasons, and optimizes sleep fragmentation in their school lives in the persepctive of adolescents behaviors and mental health, so as to find out the targeted measures of policy support, family-school cooperation and exercise support.

The expression of microRNA in nasopharyngeal carcinoma and its clinical significance / 中国医师杂志

Objective To investigate the expressions of microRNA-21,microRNA-143,and microRNA-145 in the sera of patients with nasopharyngeal carcinoma (NPC),and their assessment vales in the recurrence,metastasis,and prognosis of NPC patients.Methods From January 2012 to January 2014,80 NPC patients in our hospital Department of Internal Medicine and Head and Neck Surgery was used as tumor group,80 cases of healthy volunteers as control.The real-time fluorescence quantitative polymerase chain reaction (RT-PCR) was used to detect the expression levels of microRNA-21,microRNA-143,and microRNA-145 in the sera.Results Expression of microRNA-21 in NPC patients was significantly higher than that in healthy control group with statistically significant difference (P ＜ 0.05).The expressions of microRNA-143 and microRNA-145 in NPC patients was significantly lower than those in healthy control group with significant difference (P ＜ 0.05).At the same time,those microRNAs were significantly associated with tissue differentiation,invasion,and metastasis.Conclusions Increased microRNA-21 expression level in NPC patients,and decreased expression of microRNA-143 and microRNA-145 in NPC patients play an important role in differentiation,invasion,and metastasis in the development process of NPC.microRNAs can be used as a new index in the auxiliary diagnosis of NPC and the evaluation of recurrence,metastasis,and prognosis evaluation.

Correlation analysis of depression after stroke and ordinary status / 中国基层医药

Objective To explore the correlation of depression and ordinary status of stroke patients. Methods 260 stroke patients of our hospital from June 2012 to December 2013 were selected and divided into two groups,the observation group had depression after stroke,the control group didnˊt have depression after stroke,the ordinary status and society status of 2 groups were compared.Results Women accounted for 79.51% of the patients in the observation group,Below the level of high school education Patients accounted for 72.28%,patients with cardiovascular disease accounted for 65.06%;the patients in the observation group who was higher than 6000 yuan, accounted for 62.86%,which was lower than 86.67% of the control group (χ2 =10.21,P <0.05).The subjective support and the score of social support utilization of patients in the observation group were significantly lower than those of the control group(t =2.98,3.19,P <0.05).Conclusion Depression is a common complication for stroke patients,causing by many reasons,including gender,education level,CVD,family income and so on.

The analysis of hypocaloric nutrition in perioperative of patients with congenital hypertrophic pyloric stenosis / 重庆医学

Objective To analyze the hypocaloric nutrition(PN) in perioperative of patients with congenital hypertrophic pyloric stenosis ,and to provide reference basis for its clinical application .Methods 40 patients with congenital hypertrophic pyloric steno-sis were choseen in our hospital .All patients were divided into observation group and control group ,20 cases in each group .After the conventional support treatment ,patients in observation group were gave hypocaloric nutrition of 70 -90 kcal/kg ,patients in control group were gave hypercaloric nutrition of 100-200 kcal/kg .After 5 days ,nitrogen balance ,serum protein and body compo-sition were observed in the two groups .Results The nitrogen discharge was increased after parenteral nutrition ,after 2 days reached to peak ,and then began to decline slightly .Under the condition of the same nitrogen intake ,the nitrogen balance in observa-tion group was 10 .65 g/d ,which was significantly lower than 16 .40 g/d in control group (P0 .05) .After parenteral nutrition ,the total protein ,albumin ,prealbumin ,transferrin in observation group were (58 .22 ± 4 .63)g/L ,(43 .42 ± 3 .53)g/L ,(269 .65 ± 31 .24)g/L , (2 .83 ± 1 .23)g/L ,respectively ,which were higher than that in control group (42 .11 ± 3 .52)g/L ,(35 .57 ± 3 .82)g/L ,(252 .34 ± 27 .03)g/L ,(2 .20 ± 1 .30)g/L ,the differences were statistically significant (P0 .05) .Observation group had greater TBF and F% but decreased LBM versus control group (588 .7 ± 66 .8)g vs . (492 .8 ± 65 .6)g ,(17 .5 ± 1 .9)% vs .(15 .0 ± 1 .8)% ,(2 498 .5 ± 118 .9)g vs .(2 604 .7 ± 125 .4)g .The differences were statistical-ly significant(P<0 .05) .Conclusion Hypocaloric nutrition can meet the metabolic needs of patients with congenital hypertrophic pyloric stenosis .Hypocaloric nutrition play a positive role in improving nutritional status and can improve the postoperative rehabilitation .

Expression of nuclear factor kappaB, MCP-1 and RANTES concentration in peritoneal fluid in women with endometriosis / 实用医学杂志

Objective To compare the concentration of nuclear factor-kappa B (NF-κB), MCP-1 and RANTES in peritoneal fluid in women with endometriosis and normal peritonal fluid ,To explore the relationship between these cytokines with pathogenesis of endometriosis. Methods There were 2 groups:the endometriosis group and the control group.Double-antibody sandwich ABC-ELISA technique was adopted for determinating concentration of NF-кB、MCP-1 and RANTES in peritoneal fluid. Results Peritoneal fluid level of NF-κB MCP-1 and RANTES in endometriosis patients was higher than the control group (P < 0.05). NF-κB and MCP-1, NF-κB and RANTES expressed positive correlation by linear correlation analysis in both EMs group and control group , correlation coefficients were 0.385, 0.569(EMs group), 0.474, 0.388(P < 0.05). Conclusions NF-кB、MCP-1 and RANTES are mutual affection and promote endometriosis in the pathogenesis.

Expression of transient receptor potential canonical channel proteins in human non-small cell lung cancer / 中国肺癌杂志

<p><b>BACKGROUND AND OBJECTIVE</b>Transient receptor potential canonical (TRPC) proteins, a group of Ca2' permeable nonselective cation channels, are thought to constitute store-operated calcium channels (SOCC) and mediate store-operated calcium entry (SOCE) in various cell types. Members of TRPC have been found to be involved in abnormal proliferation, differentiation, and growth of cancer cells. The aim of this study is to detect the mRNA and protein expression of TRPC in non-small cell lung cancer (NSCLC).</p><p><b>METHODS</b>Real-time quantitative PCRwas performed to screen the expression of TRPC mRNA in NSCLC tissue. Protein expression of TRPC was detected by Western blot.</p><p><b>RESULTS</b>Among the seven family members of TRPC so far identified (TRPC1-7), we detected the expression ofTRPC1, TRPC3, TRPC4, TRPC6 mRNA in 24 cases of NSCLC tissue; TRPC2, TRPC5 and TRPC7 mRNA were not detectable. The relative abundance of the expressed TRPC was TRPC1 approximately equal TRPC6 > TRPC3 > TRPC4. Western blot confirmed the protein expression of TRPC1, TRPC3, TRPC4 and TRPC6 in NSCLC tissue.</p><p><b>CONCLUSION</b>Out of the seven members of TRPC, we found TRPC1, TRPC3, TRPC4, TRPC6 mRNA and protein were selectively expressed in human NSCLC tissue. This study could provide a basis for future exploration of the individual role of these TRPC proteins in mediating SOCE and in the progression of lung cancer.</p>

Efficacy of rapamycin drug-eluting stents in elderly patients with coronary heart disease / 中国基层医药

Objective To observe the effects and security of rapamycin drug-eluting stents implanting in eld-eay patients with coronary heart disease(CHD).Methods 107 patients with CHD treated with Firebird rapamycin drug-eluting stents,the immediate angiographic outcome,complication in hospitalization.six months'follow-up results were assessed.Results 175 Firebird stents implanted successfully,the successful stenting procedure achieved in 98.9% patients,1 patient(0.9%) died of thrombosis instent during hospitalization.there were no myocardial infarction occurred during six months'follow-up,angina recrudesced in 6 patients,and the ratio of instent restenosis was 2.6%,the MACE rate during 6 months follow-up was 2.8%.Conclusion Firebird rapamycin drug-eluting stents implanta-tion in CHD in elderly was safe and effective.

Some Reflections on Clinical Teaching Reform in Infectious Diseases / 中华医学教育探索杂志

This paper analyzes the special features of lemology education and the problems in clinical teaching at present,The measures should be taken to improve the clinical teaching quality from the varies of aspects,such as strengthening the construction of teaching staffs and students,applying the modern educational means and teaching samples in clinical teaching,launching active extracurricular activities,developing the evaluation of teaching and improving the teaching quality.