Study of the Polymorphisms rs1491033A/C and rs1813389A/G in the miR-17-92 Gene Cluster Promoter Region in a Guangxi Population / 中国医科大学学报

Objective To investigate the distribution of the polymorphisms rs1491033A/C and rs1813389A/G in the promoter region of the miR-17-92 gene cluster in a normal population of Guangxi and compare this distribution with that in different ethnic groups. Methods We detected the genotypes of rs1491033A/C and rs1813389A/G of 275 people from Guangxi using the SNaPshot technique and DNA sequencing. We then analyzed the genotype and allele frequency distribution differences among different genders and groups. Results Three genotypes,AA,AC,and CC,were found for rs1491033A/C with frequency distributions of 21. 1％,53. 1％,and 25. 8％, respectively. There were significant differences in genotype and allele frequencies of rs1491033A/C between the Guangxi population and those of Europe and Africa,as published in the International HapMap project (P < 0. 05). Three genotypes,AA,AG,and GG were observed for rs1813389A/G with frequency distributions of 40. 0％,50. 2％,and 9. 8％,respectively. There were significant differences in the genotype and allele frequencies of rs1813389A/G between the Guangxi population and those of Europe,Beijing,Japan,and Africa (P <0. 05). However,there were no significant differences in genotype or allele frequencies of the rs1491033A/C or rs1813389A/G polymorphisms between genders in the Guangxi population (P > 0. 05). Conclusion There are different distributions of the rs1491033A/C and rs1813389A/G polymorphisms in the miR-17-92 gene cluster in different races and regions.

Association of single nucleotide polymorphisms of rs3819024 and rs8193037 loci of IL-17A gene with the risk of ischemic stroke / 中华医学遗传学杂志

OBJECTIVE@#To assess the association of polymorphisms of rs3819024 and rs8193037 loci in the promoter region of IL-17A gene with the risk of ischemic stroke (IS) among ethnic Han Chinese from Guangxi.@*METHODS@#The polymorphisms of rs3819024 and rs8193037 loci were detected by a SNaPshot assay and DNA sequencing among 392 IS patients and 443 healthy controls with matched age and gender.@*RESULTS@#The genotypes, dominant model, recessive model, and alleles of rs3819024 polymorphisms showed no significant difference between the two groups, with the P values calculated as 0.150, 0.227, 0.125, 0.594 and 0.202, respectively, and OR (95% CI) as 1.27(0.92-1.74), 1.28(0.86-1.91), 1.27(0.94-1.72), 1.10(0.78-1.54), and 1.13(0.94-1.38), respectively. The genotypes, dominant model, recessive model, and alleles of rs8193037 polymorphisms also showed no significant difference between the two groups, with the P values calculated as 0.722, 0.352, 0.863, 0.345 and 0.969, respectively, and OR (95% CI) as 0.94(0.65-1.35), 2.25(0.41-12.35), 0.97(0.68-1.38), 2.27(0.41-12.48), and 1.01(0.72-1.40), respectively.@*CONCLUSION@#Polymorphisms of the rs3819024 and rs8193037 loci of the IL-17A gene are not associated with the susceptibility to IS among ethnic Han Chinese from Guangxi.

The association of Polymorphisms of Nogo gene and nasopharyngeal carcinoma in Zhuang people in Guangxi / 实用医学杂志

Objective To investigate the relationship between genetic polymorphisms of Nogo gene and nasopharyngeal carcinoma in Zhuang ethnic group in Guangxi Province.Methods Nogo gene polymorphisms were analyzed between patients with nasopharyngeal carcinoma and healthy people as a control in Zhuang ethnic group in Guangxi province using PCR-SBE.Their genotype and allele frequency distribution were compared between case and control group.The frequencies of haplotypes were analyzed with SHEsis software between these two sites in different groups.Results There were no differences between the patients and controls in the genotype or allele frequencies of Nogo gene rs 17046518 site (P ＞ 0.05).But the frequency distribution of T allele was significantly different (P =0.003) in the rs12464595 site.The result of haplotypes analysis showed that GA haplotype and CA haplotype were significantly different between cases and control (P =0.045,P =0.002).Conclusion The CG haplotype,CA haplotype and T allele of Nogo gene rs12464595 site increase the risk of nasopharyngeal carcinoma among Zhuang ethnic group in Guangxi.