Frequency survey of blood group PP1PK in Chinese Lahu Population / 中国输血杂志

【Objective】 To investigate the frequency of P1PK and GLOB blood group in Chinese Lahu population and their genetic status, so as to provide data support for the safety of blood transfusion and give advice and transfusion guidance for pregnant women. 【Methods】 Unrelated individuals of Chinese Lahu population were randomly selected for serological identification of P1PK and P blood group and gene sequencing analysis. The frequency of P1PK and GLOB blood group were analyzed. 【Results】 Six cases of anti-PP 1Pk(formerly known as anti-Tja) negative blood type were identified as the rare P1-PK-P- blood type (formerly known as Tja- blood type or p blood type, hereinafter referred to as p blood group) from 300 Lahu population, with phenotypic frequency of p blood group in P1PK and GLOB blood group system in Lahu population at 2.0%(6/300). 【Conclusion】 The phenotypic frequency of blood group p in Lahu population was significantly higher than that in Europe (5.8 persons per million) and Hong Kong, China (1 person per million),indicating significant ethnic specificity and regional ethnic differences.

Distribution of ABO, Rh, Mn, P, h blood groups and gene frequency in Lahu ethnic minority in Yunnan / 中国输血杂志

【Objective】 To understand the distribution and gene frequency of main red blood cell blood groups in Lahu ethnic minority and analyze the genetic characteristics of Lahu people. 【Methods】 1) ABO forward and reverse typing had been performed by microplate method; 2) Rh, MN, H, P1Pk and Mur antigen were tested by the tube method. If the ABO forward and reverse typing were incompatible, the tube method was used for confirmation. 【Results】 The distribution characteristics of blood group and gene frequency in Lahu ethnic minority were as follows: B>O>A>AB for ABO, with genotype frequency as p 11.1%, q 27.5% and r 61.4%; the frequency of Rh genotype was CDe 83.3%, cDE 12.0%, cDe 2.42%, CDE 2.32%, CdE 0%, Cde 0%, cdE 0% and cde 0%; M > MN>N for MN blood group, with genotype frequency as M 75.26% and N 24.74%; P1

Effect of FOCUS-PDCA program on unplanned extubation in ICU patients / 现代临床护理

Objective To observe the effect of FOCUS-PDCA on the unplanned extubation (UEX) in ICU patients. Methods About 237 ICU patients during Jan. to Dec. 2014 were assigned as the control group, where the FOCUS-PDCA program was not used. Another 235 patients during Jan. to Dec. 2015 were assigned as the study group, where the the FOCUS-PDCA program was used. The two groups were compared in view of unplanned extubation rate and nurse's comprehensive ability. Result Compared with the control group, hospitalization time was comprehensively shortened in the observation group and the nurses comprehensive abibity increased (all P<0.05). Conclusion The FOCUS-PDCA program can prevent UEX in ICU patients, reduce the rate of UEX, improve the comprehensive ability of nurses and the quality of nursing.

Differentiated thyroid cancer in children: a series of 29 cases / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To explore the best administration for the differentiated thyroid cancer in children under 14 years by reviewing of their clinical characteristics, treatment methods and results.</p><p><b>METHODS</b>Clinical data of 29 patients under 14 years with differentiated thyroid cancer in Xinhua Hospital, Shanghai Jiao Tong University School of Medicine between January 1998 and July 2014 were reviewed respectively.</p><p><b>RESULTS</b>Neck mass was the chief complaint in 27 of 29 patients. Unilateral thyroid carcinoma was found in 16 cases, and bilateral in 13 cases. Solid tumor with multiple punctate calcification was observed in 21 cases (72.4%). Cervical lymph nodes enlargement was found in 24 cases (82.8%), and 15 cases (62.5%) were bilateral. Among 20 patients received primary thyroid surgery in our hospital, 18 cases presented with T2 or advanced diseases and 16 cases had cervical lymph nodes enlargement. The resection of unilateral lobe with isthmus was performed in 2 cases, and total thyroidectomy in 18 patients, including 1 case with partial trachea resection. Neck dissection was performed in 16 patients. Of 9 patients received primary thyroid surgery in other hospitals, 8 cases presented with cervical lymph node enlargement after surgery and 6 cases with pulmonary metastasis, of them 5 patients received neck dissection, 4 patients underwent resection of residual thyroid cancer plus neck dissection. Twenty-seven of all patients were treated postoperatively with 131I. All patients received follow-up, and the meaning follow-up time was 6 years and 10 months (0.5 years-16 years). No cases with death, local recurrence, and metastasis were observed in the follow-up period.</p><p><b>CONCLUSIONS</b>Differentiated thyroid cancer is more invasive in children compared with adults. Comprehensive treatment including total thyroidectomy, neck dissection and postoperative 131I therapy may be a basic approach for the differentiated thyroid cancer in children.</p>

The spiral ganglion degeneration and the expression of EFR3A in the cochlea of the deaf mice induced by co-administration of kanamycin and furosemide / 中华耳鼻咽喉头颈外科杂志

<p><b>OBJECTIVE</b>To investigate the spiral ganglion degeneration and the expression of EFR3A in the cochlea of the deaf mice induced by co-administration of kanamycin and furosemide.</p><p><b>METHODS</b>Eight weeks old C57BL/6J mice were administered with a single dose of kanamycin followed by furosemide, then fluorescent immunohistochemistry staining and transmission electron microscopy were applied to observe the SGNs' degeneration process and extent characteristics at 1, 5, 15, 30 and 60 days following treatment. We detected the expression of EFR3A during the degeneration of SGNs via fluorescent immunohistochemistry and western blotting.</p><p><b>RESULTS</b>Co-administration of kanamycin and furosemide quickly induced cochlear hair cell death in mice, and then caused progressive degeneration of SGNs. Our results showed that the abnormal morphology of SGNs occurredat day 5 following administration, and the number of SGNs began to decrease at day 15. Compared to the control group, it was found the remarkable increase of the EFR3A protein at the fifth day after co-administration, then decreased to the nearly normal at 15 days following treatment, and no further significant changes thereafter.</p><p><b>CONCLUSION</b>The changes of the EFR3A protein expression in the spiral ganglion of the cochlea in mice are coincidence with the time of the SGNs degeneration to happen, which imply that EFR3A may play an important role in the occurrence of the SGNs' degeneration in the cochlea in mice following hair cells loss.</p>

Expression of Gult-1 in cervical adenocarcinoma and its significance / 肿瘤研究与临床

Objective To study the expression of Gult-1 in cervical adenocarcinoma and prognosis analysis.Methods Gult-1 was detected by immunohistochemistry (EnVision) in cervical adenocarcinoma and cancer adjacent tissue.Results In cancer adjacent tissue and cervical adenocarcinoma,the positive rates of Gult-1 were 2.22 ％ (1/45),58.67 ％ (44/75),respectively,and there was statistical significance between them (x2 =38.23,P =0.00).The expression of Gult-1 was not correlated to age,histological classification and infiltrated depth (all P ＞ 0.05).However,it was correlated to tumor size,histological grade and lymphatic nodes metastasis (all P ＜ 0.05).The survival rate of positive Gult-1 patients was lower than negative Gult-1 patients (x 2 =4.27,P =0.04).Conclusion The over-expression of Guh-1 in cervical adenocarcinoma indicates poorly differentiated cancer,the possibility of lymphatic nodes metastasis and unfavourable prognosis.

Effect of high-intensity focused ultrasound combined with gemcitabine on subcutaneous pancreatic cancer in nude mice / 南方医科大学学报

<p><b>OBJECTIVE</b>To investigate the effect of high-intensity focused ultrasound (HIFU) and gemcitabine on xenograft growth in nude mice bearing human pancreatic cancer.</p><p><b>METHODS</b>Nude mouse models bearing subcutaneous human pancreatic cancer cell line PANC-1 xenograft were randomized into 4 groups, including a control group and 3 treatment groups subjected to treatments with HIFU, gemcitabine, or both. After the treatments, the tumor was measured on a weekly basis for 5 weeks, and the tumor growth curve was drawn. The tumor inhibition rate was calculated and the expression of vascular endothelial growth factor (VEGF) in the tumor tissue was examined by immunohistochemistry.</p><p><b>RESULTS</b>The tumor volume showed significant differences between the 3 treatment groups (P<0.01), but all significantly smaller than that in the control group; HIFU combined with gemcitabine resulted in the most obvious reduction in the tumor volume. VEGF expression in the tumor tissue was the lowest in the combined treatment group and the highest in the control group.</p><p><b>CONCLUSION</b>HIFU therapy produces definite therapeutic effect on human pancreatic cancer in the nude mouse model, and its combination with chemotherapy is the optimal treatment modality.</p>

Expression of Twist protein, vascular endothelial growth factor mRNA and protein and their relation to biological characteristics of laryngeal squamous cell carcinom / 中国医师进修杂志

ObjectiveTo observe the expression of Twist protein, vascular endothelial growth factor (VEGF) mRNA and protein in laryngeal squamous cell carcinoma(LSCC) and their relation to clinical pathological characteristics, and aslo debate their effect and clinical application in carcinogenesis, developing of LSCC. MethodMolecular hybridization in situ was carried to detect VEGF mRNA, and immunohistochemistry S-P method was carried to detect the expression of Twist protein and VEGF protein in 80 cases of LSCC, 30 cases of paraneoplastic tissue. ResultsThe expression of Twist protein, VEGF mRNA and protein was related to TNM stage, T stage, thyroid cartilage involvement, lymph node metastasis and distance metastasis (P ＜ 0.05 or ＜ 0.01 ). The expression of Twist protein and VEGF protein was related to differentiated degree (P ＜ 0.01 or ＜ 0.05), however, the expression of VEGF mRNA was not related to differentiated degree (P ＞ 0.05 ). The expression of Twist protein was positively related to the expression of VEGF protein in LSCC (r =0.334, P ＜ 0.01 ) ; and the expression of VEGF mRNA was positive correlation to the expression of VEGF protein (r =0.484,P ＜ 0.01 ). ConclusionsOverexpression of Twist protein and VEGF mRNA and protein might play a joint action in the carcinogenesis, development of LSCC. Combined detection of Twist protein and VEGF mRNA and protein has vital significance in predicting the invasion and metastasis of LSCC, which might be an index to judge the biological behavior of LSCC.

Ultrastructural observation and quantitative analysis of apoptosis of human gastric carcinoma cell SGC-7901 induced by Jiedu-Xiaozheng decoction / 国际肿瘤学杂志

Objective To study the effect of Jiedu-xiaozheng decoction on theUltrastructure changes and quantitative analysis of SGC-7901 in vitro and its mechanisms. Methods The compound prescription Jiedu-xiaozheng decoction was used to prepare serum containing drug. Serologic pharmacology methods was applied. The decoction was used to co-culture the gastric cells for24 h and 72 h, and the ultrastructure of tumor cells was observed by the transmission electron microscope. Then the parameters of the nucleus and mitochrondria were determined in SGC-7901 cells, including volume density(Vv) ,form factor PE( PE), average volume(V) ,length(L) average surrace area(S),contrasting to cytoplasma. The SIS powervision was applied to analyze their difference. Results Jiedu-xiaozheng decotion can result in reduced microvilli, deflated volume, decreased mitochondrion volume and reduced ridge. The volume density(Vv) ,form factor PE(PE) ,average volume(v) ,length (L) average surrace area(S) reduced in the 24 h group clearly in Jiedu-xiaozheng decoction group compared to control group. Conclusion Jiedu-xiaozheng decoction can significantly induce SGC-7901 cells apoptosis, exhibiting certain early ultrastructure changes such as marginated heterochromatin and mitochondrion pyknosis. The quantity analysis by electronic imaging system may objectively reveal the ultrastructural changes of cell nucleus and mitochondria.

Glutaral Solution Conternts Determined by Enzyme-labeled Device / 中华医院感染学杂志

OBJECTIVE To study the analytic method for assaying of glutaral concentration by enzyme-labeled device at one time.METHODS Controlled solution of glutaral and sample solution were taken out and kept until the room temperature.Optical density value was detected at 450 nm.Content of sample was calculated by comparison with the known concentration of glutaral.RESULTS In the range of 0.75-2.50%,concentration of glutaral had linear relationship with the optical density value.Reproductive test:relative average error was within 2%,RSD≤2.51%;interference test:oxidation-resistant-sodium nitrite could make the result negative error.0.1% Sodium nitrite could make the test result decrease by 1.5% relatively.CONCLUSIONS The method is simple,fast and convenient,with accuracy which has met the requirement of hospital infection monitoring and acceptable interfering range made by additives,which is highly practical.

Expression of Fascin-1 Protein in Colorectal Adenocarcinoma and Relation with Its Clinicopathologic Characteristics / 中国普外基础与临床杂志

Objective To investigate the expression of Fascin-1 protein in colorectal adenocarcinoma,and the relationship with its clinicopathologic features.Methods The expressions of Fascin-1 protein in colorectal adenocarcinoma tissues of 60 cases,colorectal adenoma tissues of 30 cases and normal mucosa tissues (4 cm distance to neoplasm) of 30 cases were detected by Microwave-EliVisionTM immunohistochemistry method,and the relationship between the expression of Fascin-1 protein in colorectal adenocarcinoma tissues and its clinicopathologic characteristics was analyzed.Results The expression of Fascin-1 protein was located in cytoplasm.The positive expression rates of Facsin-1 protein were 3.3% (1/30),30.0% (9/30) and 53.3% (32/60) in normal mucosa tissues,colorectal adenoma tissues and colorectal adenocarcinoma tissues,respectively.The expression of Fascin-1 was gradually increased in these three tissues,and there was statistical difference among the three tissues (P0.05).Conclusion The high expression of Fascin-1 protein is correlated to high invasion ability and lymph node metastasis,which can play as a sensitive index in predicting the invasion and metastasis of colorectal adenocarcinoma.

Quality control of Compound Glucomannan Weight-reducing Tablet / 中成药

AIM:To establish the quality control standard of Compound Glucomannan Weight-reducing Tablet. METHODS:Material and tablets went through ultrasonic cleaning by 60% alcohol and then hydrolyzed to glucose and mannose with 2.0 mol/L sulfuric acid,derivatized with PMP,separated by RP-HPLC using a stage gradient elution,and wavelength was at 250 nm. The monosaccharide were studied under the help of internal standard; while the glucomannan content in Konjac refined powder and tablets was determined. RESULTS:Linearities of glucose and mannose were good (r=0.994 3,0.999 8) in the range of 0.05-0.5 mmol/L.The average recoveries of this method were 100.67% and 98.34%,RSD were 2.74% and 3.22%(n=5) respectively. Ratio of glucose to mannose in glucomannan was 2 ∶3,glucomannan content in Konjac refined powder was 88.2%,glucomannan content in tablet was 354 mg/p. dissolution used for glucomannan conformed to homogeneity. CONCLUSION:The established quality standard is simple,feasible and reproducible,and can be used for quality control of Compound Glucomannan Weight-reducing Tablet.