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Objective@#Schizophrenia is a serious mental disorder. Mutations in mitochondrial genes can change energy metabolism. Telomere is a tandem sequence at the end of chromosomes. Shorter telomere length has been shown in schizophrenia. The aim of this study was to determine the relationship between ATPase6 gene mutations and telomere length in schizophrenia patients. @*Methods@#Blood samples of 34 patients and 34 healthy controls were used. In this study conventional PCR, Sanger sequencing technic and real-time PCR were utilized. @*Results@#Five different mutations (A8860G, A8836, G8697A, C8676T, and A8701G) in the ATPase6 gene were identified in schizophrenia patients. The most seen mutation was A8860G (94%). Telomere length analysis indicated the relation of ATPase6 gene mutations and telomere length variations (p = 0.001). Patients carrying the A8860G mutation had shorter telomere lengths than patients carrying other mutations. Comparing telomere length between schizophrenia patients and healthy controls revealed that the mean telomere length of schizophrenia patients was shorter than healthy controls (p = 0.006). The demographic analysis demonstrated a significant relationship between marital status and telomere length (p = 0.011). Besides that, the duration of the illness is another factor that impacts telomere length (p = 0.044). There is no significant relation between telomere length and other clinical and demographic characteristics including education status, age, gender, etc. @*Conclusion@#In conclusion, telomere length and ATPase6 gene mutations have a significant relation. Studies with larger patient populations and investigation of other mitochondrial gene mutations will make the clearer link between telomere length and mitochondrial mutations.
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Objective@#Apoptosis is programmed cell death that occurs by several pathways. Caspase-3 is induced by active caspase-9 via the intrinsic pathway. The aim of this research was to explore the expression of caspase-3 and caspase-9 in schizophrenia patients and healthy samples. @*Methods@#RNA was isolated from the peripheral blood of 39 schizophrenia patients’ and healthy samples. After cDNA synthesis, real time PCR (RT-PCR) was used to analyse caspase-3 and caspase-9 gene expression. The severity of psychopathological symptoms of schizophrenia was evaluated using the Positive and Negative Symptoms Scale for schizophrenia (PANSS) and Clinical Global Impressions (CGI). @*Results@#The expression of caspase-3 and caspase-9 genes was higher in schizophrenia patients than in healthy samples (p = 0.012, p = 0.002, respectively). The increase in caspase-3 gene expression was significant with being male, smoking and with a duration of less than 6 years (p = 0.047, p = 0.049, p = 0.034, respectively). On the other hand, the increase in caspase-9 gene expression was significant in patients who is smoke, have children, and are under 33 years old (p = 0.040, p = 0.043, p = 0.045, respectively). A significant positive correlation was detected between the caspase-3 and caspase-9 gene expression (r = 0.3218, p = 0.049). @*Conclusion@#Our findings indicate that caspase-3 and caspase-9 gene expression may activate cell death mechanisms by intrinsic apoptotic genes. Furthermore, caspase-3 and caspase-9 may play essential roles in different ways in schizophrenia. Hence there is a need to further study the apoptotic mechanism with expanded patient populations.
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ABSTRACT: The agriculture sector which has gained importance with Industry 4.0 in recent years, strives to adapt to the needs and requirements of the era by stripping from its traditional structure. In this process, it is important to increase the motivation (intrinsic and extrinsic) of human resources factor, which is the most important input of agriculture, on innovation, creativity and entrepreneurship. This paper examined the factors that affect the intrinsic motivation of the agricultural holdings managers in Kirsehir province of Turkey on their creativity for innovation, which is one of the main variables of growth. Questionnaire forms were filled with 312 agricultural holdings managers who were selected according to the Probability Sampling Method in September-October, 2019. Intrinsic motivation scores for creativity (IMC) were obtained by using 5-point scale. Determined some socio-demographical, agricultural, economic and behavioral variables for agricultural holdings were tested with IMC levels. As a result of the study, it was determined that variables such as education, social security status, share of agricultural income, subjective poverty and risk behaviors significantly affect the IMC level of the agricultural enterprise managers. As a result, the measures to increase both the internal and external motivations of the agricultural enterprises managers are required in order to make agriculture more competitive, profitable and sustainable by removing it from the traditional structure. In this regard, not only the economic side but also the social side of agriculture should not be ignored and the potential of agriculture should be used more with structural reforms.
RESUMO: O setor agrícola, que ganhou importância com a Indústria 4.0 nos últimos anos, esforça-se por se adaptar às necessidades e exigências da época retirando sua estrutura tradicional. Nesse processo é importante aumentar a motivação do fator recursos humanos, que é o insumo mais importante da agricultura, em inovação, criatividade e empreendedorismo. Com este estudo, tentou-se revelar os fatores que afetam a motivação dos gerentes de empresas agrícolas na província de Kirsehir, na Turquia, sobre inovação e criatividade, que é uma das principais variáveis de crescimento. Os formulários do questionário foram preenchidos com 312 gerentes de empresas agrícolas, selecionados de acordo com o Método de Amostragem por Probabilidade. Os escores de motivação intrínseca para a criatividade (IMC) foram obtidos usando a escala de cinco pontos. Determinou-se que algumas variáveis sociodemográficas, agrícolas, econômicas e comportamentais para explorações agrícolas foram testadas com os níveis de IMC. Como resultado do estudo, foi determinado que variáveis como educação, status de seguridade social, parcela da renda agrícola, pobreza subjetiva e comportamentos de risco afetam significativamente o nível de IMC dos gerentes de empresas agrícolas. Como resultado, são necessárias medidas para aumentar as motivações internas e externas dos gerentes de empresas agrícolas, a fim de tornar a agricultura mais competitiva, rentável e sustentável, removendo-a da estrutura tradicional. Nesse sentido, não apenas o lado econômico, mas também o lado social da agricultura não devem ser ignorados e o potencial da agricultura deve ser mais utilizado nas reformas estruturais.